Cloned (Comment) | Organism |
---|---|
overexpression in Streptomyces lividans | Streptomyces glaucescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
tetracenomycin A2 | pH 9.0, 25°C | Streptomyces glaucescens | |
0.082 | - |
NADH | pH 9.0, 25°C | Streptomyces glaucescens | |
0.26 | - |
NADPH | pH 9.0, 25°C | Streptomyces glaucescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000, SDS-PAGE | Streptomyces glaucescens |
61000 | - |
gel filtration | Streptomyces glaucescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+ | Streptomyces glaucescens | biosynthesis tetracenomycin C, a polyketide antitumor antibiotic | tetracenomycin C + 2 NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces glaucescens | Q7M0K6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces glaucescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00429 | - |
pH 9.0, 25°C | Streptomyces glaucescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+ | biosynthesis tetracenomycin C, a polyketide antitumor antibiotic | Streptomyces glaucescens | tetracenomycin C + 2 NAD(P)+ + H2O | - |
? | |
tetracenomycin A2 + 2 O2 + 2 NADH + 2 H+ | tetracenomycin A2 i.e. methyl 10,12-dihydroxy-3,8-dimethoxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate. The enzyme prefers NADPH over NADH as an electron donor | Streptomyces glaucescens | tetracenomycin C + 2 NAD+ + H2O | tetracenomycin C = methyl (6aR,7S,10aR)-6a,7,10a,12-tetrahydroxy-3,8-dimethoxy-1-methyl-6,10,11-trioxo-6,6a,7,10,10a,11-hexahydrotetracene-2-carboxylate | ? | |
tetracenomycin A2 + 2 O2 + 2 NADPH + 2 H+ | tetracenomycin A2 i.e. methyl 10,12-dihydroxy-3,8-dimethoxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate. The enzyme prefers NADPH over NADH as an electron donor | Streptomyces glaucescens | tetracenomycin C + 2 NADP+ + H2O | tetracenomycin C = methyl (6aR,7S,10aR)-6a,7,10a,12-tetrahydroxy-3,8-dimethoxy-1-methyl-6,10,11-trioxo-6,6a,7,10,10a,11-hexahydrotetracene-2-carboxylate | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Streptomyces glaucescens |
Synonyms | Comment | Organism |
---|---|---|
ElmG | - |
Streptomyces glaucescens |
Tcm A2 oxygenase | - |
Streptomyces glaucescens |
TcmG | - |
Streptomyces glaucescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | in 50 mM Tris-HC1 or 50 nm ethanolamine-HCl buffer | Streptomyces glaucescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | pH 7.5: about 45% of maximal activity, pH 10: about 25% of maximal activity | Streptomyces glaucescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 mol of non-covalently bound FAD. The apoenzyme can be partially reconstituted in vitro by addition of FAD | Streptomyces glaucescens |