Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | two flavin conformations in the enzyme: the in-position and the out-position. Substrate hydroxylation occurs while the flavin in the enzyme is in the in-conformation. Flavin must move to the out-conformation for proper formation of the charge-transfer complex between NADPH and FAD that is necessary for rapid flavin reduction | Pseudomonas aeruginosa | |
FAD | thermodynamic and kinetic constants of the enzyme reconstituted with 8-substituted flavins | Pseudomonas aeruginosa |