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Literature summary for 1.14.13.195 extracted from

  • Romero, E.; Fedkenheuer, M.; Chocklett, S,W.; Qi, J.; Oppenheimer, M.; Sobrado, P.
    Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase (2012), Biochim. Biophys. Acta, 1824, 850-857.
    View publication on PubMed
Search for more literature for 1.14.13.195

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 NADPH pH 7.5, 15°C Aspergillus fumigatus
0.1
-
 NADH pH 7.5, 15°C Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q5SE95
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + NADH + H+ + O2
-
 Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2
-
 Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
additional information when the enzyme is reduced with NADPH and reacts with molecular oxygen, a C4a-hydroperoxyflavin intermediate is observed. When the enzyme is reduced with NADH, the intermediate is 2fold less stable. Steady-state kinetic isotope effect values for NADPH and NADH are 3 and 2 , respectively, due to differences in the rate of flavin reduction by these coenzymes. NADP+, and not NAD+, protects the enzyme from proteolysis, suggesting that it induces conformational changes upon binding Aspergillus fumigatus ?
-
 ?

Synonyms

Synonyms Comment Organism
SidA
-
 Aspergillus fumigatus
siderophore A
-
 Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 NADPH pH 7.5, 15°C Aspergillus fumigatus
0.73
-
 NADH pH 7.5, 15°C Aspergillus fumigatus

General Information

General Information Comment Organism
physiological function enzyme is essential for virulence Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 NADH pH 7.5, 15°C Aspergillus fumigatus
50
-
 NADPH pH 7.5, 15°C Aspergillus fumigatus