Activating Compound | Comment | Organism | Structure |
---|---|---|---|
6-hydroxy-3-succinoylpyridine | acts as an initial effector and strongly stimulates NADH oxidation | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of a GADGA motif in the FAD binding-domain results in complete loss of activity in the mutant protein | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.104 | - |
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate | pH 8.0, 25°C | Pseudomonas putida | |
0.175 | - |
NADH | pH 8.0, 25°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2 mM, complete loss of activity | Pseudomonas putida | |
Zn2+ | 2 mM, complete loss of activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Pseudomonas putida |
80000 | - |
PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | F8G0M4 | - |
- |
Pseudomonas putida S16 | F8G0M4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.8 | - |
pH 8.0, 25°C | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, stable for several months | Pseudomonas putida |
4°C Tris-HCl buffer, stable for at least one week | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | - |
Pseudomonas putida | 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? | |
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | - |
Pseudomonas putida S16 | 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
HSP hydroxylase | - |
Pseudomonas putida |
hspB | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
30 min, stable up to | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
NADH | pH 8.0, 25°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | incubation overnight, 20% loss of activity | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas putida | |
additional information | no cofactor: NADPH | Pseudomonas putida | |
NADH | specific for NADH | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
physiological function | gene is essential for nicotine degradation | Pseudomonas putida |