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Literature summary for 1.14.13.154 extracted from

  • Montemiglio, L.C.; Gianni, S.; Vallone, B.; Savino, C.
    Azole drugs trap cytochrome P450 EryK in alternative conformational states (2010), Biochemistry, 49, 9199-9206.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant His-tagged EryK is overexpressed in BL21 STAR (DE3) Escherichia coli strain Saccharopolyspora erythraea

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of EryK in complex with the azole inhibitors ketoconazole and clotrimazole Saccharopolyspora erythraea

Inhibitors

Inhibitors Comment Organism Structure
clotrimazole induces a distortion of the internal helix I that affects the accessibility of the binding pocket by regulating the kink of the external helix G via a network of interactions that involves helix F Saccharopolyspora erythraea
ketoconazole induces a distortion of the internal helix I that affects the accessibility of the binding pocket by regulating the kink of the external helix G via a network of interactions that involves helix F Saccharopolyspora erythraea

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea P48635
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharopolyspora erythraea

Synonyms

Synonyms Comment Organism
EryK
-
Saccharopolyspora erythraea

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 a heme-thiolate protein (P-450) Saccharopolyspora erythraea