Cloned (Comment) | Organism |
---|---|
gene shy1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis | Fusarium graminearum |
gene shyC, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis | Fusarium graminearum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a disruption mutant of Fusarium graminearum strain NRRL 46422 gene shyC, replacement of FgShyC is confirmed by genome sequencing and analysis | Fusarium graminearum |
additional information | generation of enzyme deletion mutants. The wild-type strain PH-1 degrades approximately 80% of the added salicylate (SA) whereas Fgshy1 deletion mutants are significantly reduced in their ability to degrade SA in liquid culture. Compared to media control, Fgshy1-M1 and M2 mutants are able to degrade 10 and 11% of the SA, respectively, but mutant Fgshy1-M18 is almost inactive. With addition of SA at concentrations of 1 and 2 mM, the Fgshy1 mutant grows slightly slower than the wild-type PH-1 and the hyphae turn yellow. But no significant growth difference between Fgshy1 mutant and PH-1 is found by statistical analysis. Disruption of FgShy1 does not significantly affect Fusarium graminearum | Fusarium graminearum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
salicylate + NADH + 2 H+ + O2 | Fusarium graminearum | - |
catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | Fusarium graminearum NRRL 31084 | - |
catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | Fusarium graminearum CBS 123657 | - |
catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | Fusarium graminearum PH-1 | - |
catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | Fusarium graminearum ATCC MYA-4620 | - |
catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | Fusarium graminearum FGSC 9075 | - |
catechol + NAD+ + H2O + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium graminearum | A0A455ZJ87 | Fusarium graminearum | - |
Fusarium graminearum | I1RIL9 | Fusarium graminearum | - |
Fusarium graminearum ATCC MYA-4620 | I1RIL9 | Fusarium graminearum | - |
Fusarium graminearum CBS 123657 | I1RIL9 | Fusarium graminearum | - |
Fusarium graminearum FGSC 9075 | I1RIL9 | Fusarium graminearum | - |
Fusarium graminearum NRRL 31084 | I1RIL9 | Fusarium graminearum | - |
Fusarium graminearum NRRL 46422 | A0A455ZJ87 | Fusarium graminearum | - |
Fusarium graminearum PH-1 | I1RIL9 | Fusarium graminearum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the ability of wild-type from strain 46422 and the FgshyC mutant to metabolize salicylate (SA) is tested. When SA is added to liquid cultures of either wild-type strain 46422 or the FgShyC mutant, it is degraded similarly. This demonstrates that Fusarium graminearum produces salicylate hydroxylases and argues against this role for FgShyC. In addition, recombinant FgShyC protein expressed in Escherichia coli does not convert SA to catechol in a colorimetric plate assay. Although FgShyC has sequence homology with salicylic hydroxylase genes, it does not function as a salicylate hydroxylase in our assays | Fusarium graminearum | ? | - |
- |
|
additional information | the ability of wild-type from strain 46422 and the FgshyC mutant to metabolize salicylate (SA) is tested. When SA is added to liquid cultures of either wild-type strain 46422 or the FgShyC mutant, it is degraded similarly. This demonstrates that Fusarium graminearum produces salicylate hydroxylases and argues against this role for FgShyC. In addition, recombinant FgShyC protein expressed in Escherichia coli does not convert SA to catechol in a colorimetric plate assay. Although FgShyC has sequence homology with salicylic hydroxylase genes, it does not function as a salicylate hydroxylase in our assays | Fusarium graminearum NRRL 46422 | ? | - |
- |
|
salicylate + NADH + 2 H+ + O2 | - |
Fusarium graminearum | catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | - |
Fusarium graminearum NRRL 31084 | catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | - |
Fusarium graminearum CBS 123657 | catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | - |
Fusarium graminearum PH-1 | catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | - |
Fusarium graminearum ATCC MYA-4620 | catechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + 2 H+ + O2 | - |
Fusarium graminearum FGSC 9075 | catechol + NAD+ + H2O + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD_binding_3 domain-containing protein | UniProt | Fusarium graminearum |
FGSG_03657 | - |
Fusarium graminearum |
FgShy1 | - |
Fusarium graminearum |
FgShyC | - |
Fusarium graminearum |
SA hydroxylase | - |
Fusarium graminearum |
salicylate hydroxylase | - |
Fusarium graminearum |
Shy1 | - |
Fusarium graminearum |
ShyC | - |
Fusarium graminearum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Fusarium graminearum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Fusarium graminearum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Fusarium graminearum | |
NADH | - |
Fusarium graminearum |
Organism | Comment | Expression |
---|---|---|
Fusarium graminearum | the enzyme is highly induced by salicylate. FgShy1 expression is induced about fourfold during wheat head infection | up |
Fusarium graminearum | the enzyme is only very slightly induced by salicylate | up |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis and tree, salicylate hydroxylase homologue FgShy1 is most closely related to NahG from Pseudomonas putida and Shy1 from Ustilago maydis | Fusarium graminearum |
evolution | phylogenetic analysis and tree, salicylate hydroxylase homologue FgShyC is unique to the North American population 2 (NA2), FgShyC is present only in NA2 strains | Fusarium graminearum |
physiological function | FgShy1 is not essential for the growth of Fusarium graminearum on agar medium with SA, suggesting additional enzymes or other SA degradation pathways exist | Fusarium graminearum |