Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.12.23 extracted from

  • Keenan, B.G.; Leungsakul, T.; Smets, B.F.; Mori, M.A.; Henderson, D.E.; Wood, T.K.
    Protein engineering of the archetypal nitroarene dioxygenase of Ralstonia sp. strain U2 for activity on aminonitrotoluenes and dinitrotoluenes through alpha-subunit residues leucine 225, phenylalanine 350, and glycine 407 (2005), J. Bacteriol., 187, 3302-3310.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.23

Protein Variants

Protein Variants Comment Organism
F350T contrary to wild-type, mutant produces 3-methyl-4-nitrocatechol from 2,6-dinitrotoluene and 3-amino-4-methyl-5-nitrocatechol and 2-amino-4,6-dinitrobenzyl alcohol from 2-amino-4,6-dinitrotoluene, and mutant releases nitrite from 2,3-dinitrotoluene sixfold faster than wild-type Ralstonia sp.
F350T/G407S double mutant increases the rate of oxidation of 2,3-dinitrotoluene, 2,6-dinitrotoluene , and 2-amino-4,6-dinitrotoluene threefold relative to variant F350T Ralstonia sp.
G50S/L225R/A269T mutant displays increased rate of 4-amino-2-nitrotoluene oxidation and additionally produces oxidation product 4-amino-2-nitrocresol (enhanced 11fold relative to wild-type) as well as 4-amino-2-nitrobenzyl alcohol Ralstonia sp.
L225R 12fold faster generation of 4-amino-2-nitrocresol and production of 4-amino-2-nitrobenzyl alcohol from 4-amino-2-nitrotoluene as well as 24fold faster generation of nitrite and 15fold faster generation of 2,3-dinitrobenzyl alcohol from 2,3-dinitrotoluene Ralstonia sp.

Organism

Organism UniProt Comment Textmining
Ralstonia sp.
-
-
-
Ralstonia sp. U2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dinitrotoluene + NADH + O2 no substrate for wild-type, but substrate of mutant F350T Ralstonia sp. 4-methyl-3-nitrocatechol + nitrite + NAD+
-
 ?
2,3-dinitrotoluene + NADH + O2 no substrate for wild-type, but substrate of mutant F350T Ralstonia sp. U2 4-methyl-3-nitrocatechol + nitrite + NAD+
-
 ?
2,6-dinitrotoluene + NADH + O2 no substrate for wild-type, but substrate of mutant F350T Ralstonia sp. 3-methyl-4-nitrocatechol + nitrite + NAD+
-
 ?
2,6-dinitrotoluene + NADH + O2 no substrate for wild-type, but substrate of mutant F350T Ralstonia sp. U2 3-methyl-4-nitrocatechol + nitrite + NAD+
-
 ?
2-amino-4,6-dinitrotoluene + NADH + O2 no substrate for wild-type, but substrate of mutant F350T Ralstonia sp. 3-amino-4-methyl-5-nitrocatechol + nitrite + NAD+ plus 2-amino-4,6-dinitrobenzyl alcohol ?
2-amino-4,6-dinitrotoluene + NADH + O2 no substrate for wild-type, but substrate of mutant F350T Ralstonia sp. U2 3-amino-4-methyl-5-nitrocatechol + nitrite + NAD+ plus 2-amino-4,6-dinitrobenzyl alcohol ?
additional information wild-type enzyme catalyzes dioxygenation of naphthalen, reaction of EC 1.14.12.12 Ralstonia sp. ?
-
 ?
additional information wild-type enzyme catalyzes dioxygenation of naphthalen, reaction of EC 1.14.12.12 Ralstonia sp. U2 ?
-
 ?

Synonyms

Synonyms Comment Organism
naphthalene dioxygenase
-
 Ralstonia sp.