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Literature summary for 1.14.12.13 extracted from

  • Romanov, V.; Hausinger, R.P.
    Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate (1994), J. Bacteriol., 176, 3368-3374.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.13

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulation, 1.64 mol per mol of enzyme Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa 142
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.57
-
 30°C, pH 6.5 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorobenzoate + NADH + O2 50% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 4-chlorocatechol + chloride + NAD+ + CO2
-
 ?
2,4-dichlorobenzoate + NADH + O2 50% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 142 4-chlorocatechol + chloride + NAD+ + CO2
-
 ?
2,5-dichlorobenzoate + NADH + O2 38% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 4-chlorocatechol + chloride + NAD+ + CO2
-
 ?
2,5-dichlorobenzoate + NADH + O2 38% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 142 4-chlorocatechol + chloride + NAD+ + CO2
-
 ?
2,6-dichlorobenzoate + NADH + O2 19% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa ? + NAD+ + CO2
-
 ?
2-amino-4-chlorobenzoate + NADH + O2 28% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa ? + NAD+ + CO2
-
 ?
2-amino-5-chlorobenzoate + NADH + O2 27% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa ? + NAD+ + CO2
-
 ?
2-bromobenzoate + NADH + O2 107% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa catechol + bromide + NAD+ + CO2
-
 ?
2-bromobenzoate + NADH + O2 107% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 142 catechol + bromide + NAD+ + CO2
-
 ?
2-chloro-5-bromobenzoate + NADH + O2 50% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 4-bromocatechol + chloride + NAD+ + CO2
-
 ?
2-chlorobenzoate + NADH + H+ + O2
-
 Pseudomonas aeruginosa catechol + chloride + NAD+ + CO2
-
 ?
2-chlorobenzoate + NADH + H+ + O2
-
 Pseudomonas aeruginosa 142 catechol + chloride + NAD+ + CO2
-
 ?
2-iodobenzoate + NADH + O2 130% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa catechol + iodide + NAD+ + CO2
-
 ?
2-iodobenzoate + NADH + O2 130% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 142 catechol + iodide + NAD+ + CO2
-
 ?
3,4-dichlorobenzoate + NADH + O2 10% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa ? + NAD+ + CO2
-
 ?
3,5-dichlorobenzoate + NADH + O2 16% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa ? + NAD+ + CO2
-
 ?
3-chlorobenzoate + NADH + H+ + O2 15% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa catechol + chloride + NAD+ + CO2
-
 ?
4-amino-2-chlorobenzoate + NADH + O2 21% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 4-aminocatechol + chloride + NAD+ + CO2
-
 ?
4-chlorobenzoate + NADH + H+ + O2 21% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa catechol + chloride + NAD+ + CO2
-
 ?
benzoate + NADH + H+ + O2 37% of the activity with 2-chlorobenzoate Pseudomonas aeruginosa 3,5-cyclohexadiene-1,2-diol + NAD+ + CO2
-
 ?

Subunits

Subunits Comment Organism
More N-terminal amino acid sequence Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas aeruginosa
additional information NADPH may not substitute for NADH Pseudomonas aeruginosa
NADH
-
 Pseudomonas aeruginosa