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Literature summary for 1.14.12.12 extracted from

  • Jouanneau, Y.; Meyer, C.; Jakoncic, J.; Stojanoff, V.; Gaillard, J.
    Characterization of a naphthalene dioxygenase endowed with an exceptionally broad substrate specificity toward polycyclic aromatic hydrocarbons (2006), Biochemistry, 45, 12380-12391.
    View publication on PubMed
Search for more literature for 1.14.12.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pseudomonas putida Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00042
-
 biphenyl at 30°C in reaction mixtures containing 0.00013 mM ht-PhnI and 0.5 mM NADH in 50 mM potassium phosphate buffer, pH 7.0 Sphingomonas sp.
0.00092
-
 Naphthalene at 30°C in reaction mixtures containing 0.00013 mM ht-PhnI and 0.5 mM NADH in 50 mM potassium phosphate buffer, pH 7.0 Sphingomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the iron content of the oxygenase component ht-PhnI varies between 1.73 and 2.55 Fe atoms per pair of subunits depending on preparation, preincubation of ht-PhnI with ferrous ions under reducing conditions prior to enzyme assay results in a marginal increase of activity, the iron content of the ferredoxin component ht-PhnA3 is estimated to be 1.5 mol/mol of ferredoxin Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13500
-
 ferredoxin component, gel filtration Sphingomonas sp.
20000
-
 3 * 52000, 3 * 20000, alpha3beta3 hexamer, oxygenase component, SDS-PAGE Sphingomonas sp.
45000
-
 reductase component, gel filtration Sphingomonas sp.
52000
-
 3 * 52000, 3 * 20000, alpha3beta3 hexamer, oxygenase component, SDS-PAGE Sphingomonas sp.
200000
-
 oxydase component, gel filtration Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose DE52 column chromatography, immobilized metal affinity chromatography and DEAE cellulose column chromatography Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 benz[a]anthracene + 3 NADH + 3 O2 + 3 H+
-
 Sphingomonas sp. benz[a]anthracene-10,11-cis-dihydrodiol + benz[a]anthracene-8,9-cis-dihydrodiol + benz[a]anthracene-1,2-cis-dihydrodiol + 3 NAD+
-
 ?
benz[a]anthracene-1,2-cis-dihydrodiol + NADH + O2
-
 Sphingomonas sp. benz[a]anthracene-1,2,10,11-bis-cis-dihydrodiol + NAD+
-
 ?
benz[a]anthracene-10,11-cis-dihydrodiol + NADH + O2
-
 Sphingomonas sp. benz[a]anthracene-1,2,10,11-bis-cis-dihydrodiol + NAD+
-
 ?
biphenyl + NADH + O2
-
 Sphingomonas sp. ? + NAD+
-
 ?
chrysene + NADH + O2
-
 Sphingomonas sp. chrysene-3,4-cis-dihydrodiol + chrysene-3,4,9,10-bis-cis-dihydrodiol + NAD+
-
 ?
naphthalene + NADH + O2
-
 Sphingomonas sp. (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
phenanthrene + NADH + O2
-
 Sphingomonas sp. ? + NAD+
-
 ?

Subunits

Subunits Comment Organism
hexamer 3 * 52000, 3 * 20000, alpha3beta3 hexamer, oxygenase component, SDS-PAGE Sphingomonas sp.
monomer 1 * 13500, ferredoxin component, gel filtration Sphingomonas sp.

Synonyms

Synonyms Comment Organism
naphthalene dioxygenase the enzyme is a three-component dioxygenase complex consisting of ht-PhnI (oxygenase component), ht-PhnA3 (ferredoxin component), and ht-PhnA4 (reductase component) Sphingomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.01
-
 biphenyl at 30°C in reaction mixtures containing 0.00013 mM ht-PhnI and 0.5 mM NADH in 50 mM potassium phosphate buffer, pH 7.0 Sphingomonas sp.
1.82
-
 Naphthalene at 30°C in reaction mixtures containing 0.00013 mM ht-PhnI and 0.5 mM NADH in 50 mM potassium phosphate buffer, pH 7.0 Sphingomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Sphingomonas sp.