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Literature summary for 1.14.12.10 extracted from

  • Tavakoli, A.; Hamzah, A.; Rabu, A.
    Expression, purification and kinetic characterization of recombinant benzoate dioxygenase from Rhodococcus ruber UKMP-5M (2016), Mol. Biol. Res. Commun., 5, 133-142 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus ruber

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00558
-
benzoate at pH 8.5 and 25°C Rhodococcus ruber

Organism

Organism UniProt Comment Textmining
Rhodococcus ruber
-
-
-
Rhodococcus ruber UKMP-5M
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi Trap SP-FF Sepharose column chromatography Rhodococcus ruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene + NADH + H+ + O2
-
Rhodococcus ruber ?
-
?
benzene + NADH + H+ + O2
-
Rhodococcus ruber UKMP-5M ?
-
?
benzoate + NADH + H+ + O2
-
Rhodococcus ruber ?
-
?
benzoate + NADH + H+ + O2
-
Rhodococcus ruber UKMP-5M ?
-
?
butyl benzol + NADH + H+ + O2
-
Rhodococcus ruber ?
-
?
butyl benzol + NADH + H+ + O2
-
Rhodococcus ruber UKMP-5M ?
-
?
ethyl benzene + NADH + H+ + O2 highest activity Rhodococcus ruber ?
-
?
ethyl benzene + NADH + H+ + O2 highest activity Rhodococcus ruber UKMP-5M ?
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Rhodococcus ruber

Synonyms

Synonyms Comment Organism
BenA
-
Rhodococcus ruber

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Rhodococcus ruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Rhodococcus ruber

Cofactor

Cofactor Comment Organism Structure
NADH
-
Rhodococcus ruber

pI Value

Organism Comment pI Value Maximum pI Value
Rhodococcus ruber isoelectric focusing
-
10.4