Application | Comment | Organism |
---|---|---|
synthesis | Synthesis of useful chiral chemical products: biotransformation of benzoate to cis-diols | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
The genes benABC encoding benzoate dioxygenase are cloned into plasmid pSYM01 which harboring a cis-diol dehydrogenase knockout and overexpressed in Pseudomona putida KTSY01. | Pseudomonas putida |
General Stability | Organism |
---|---|
Pseudomonas putida may be one of these bacteria that exhibits a strong tolerance to the toxicity of benzoate. | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoate + NADH + H+ + O2 | Pseudomonas putida | - |
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ | - |
? | |
benzoate + NADH + H+ + O2 | Pseudomonas putida | - |
cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+ | Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield). | ? | |
benzoate + NADH + H+ + O2 | Pseudomonas putida KT 2240 | - |
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ | - |
? | |
benzoate + NADH + H+ + O2 | Pseudomonas putida KT 2240 | - |
cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+ | Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield). | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
and strain KTSY01 | - |
Pseudomonas putida KT 2240 | - |
and strain KTSY01 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
The recombinant bacteria shows a BZDO activity of 1.2 mmol benzoate per gram cell dry weight, while Pseudomonas putida KTSY01 shows a BZDO activity of only 0.09 mmol. | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoate + NADH + H+ + O2 | - |
Pseudomonas putida | (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ | - |
? | |
benzoate + NADH + H+ + O2 | - |
Pseudomonas putida KT 2240 | (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ | - |
? | |
benzoate + NADH + H+ + O2 | - |
Pseudomonas putida | cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+ | Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield). | ? | |
benzoate + NADH + H+ + O2 | - |
Pseudomonas putida KT 2240 | cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+ | Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield). | ? |
Synonyms | Comment | Organism |
---|---|---|
benABC | genes are organized in a single cluster and are under benzoate induced regulation. The benABC genes jointly encode benzoate 1,2-dioxygenase, which catalyzes the dihydroxylation of benzoate to produce 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid | Pseudomonas putida |
benzoate 1,2-dioxygenase | - |
Pseudomonas putida |
benzoate 1,2-dioxygenase system | BZDOS | Pseudomonas putida |
benzoate dioxygenase | - |
Pseudomonas putida |
BZDO enzyme | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas putida |