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Literature summary for 1.14.11.B16 extracted from

  • Hannibal, L.; Lorquin, J.; D'Ortoli, N.A.; Garcia, N.; Chaintreuil, C.; Masson-Boivin, C.; Dreyfus, B.; Giraud, E.
    Isolation and characterization of canthaxanthin biosynthesis genes from the photosynthetic bacterium Bradyrhizobium sp. strain ORS278 (2000), J. Bacteriol., 182, 3850-3853.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate activation Bradyrhizobium sp.
ascorbate activation Bradyrhizobium sp.
catalase activation Bradyrhizobium sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bradyrhizobium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activation Bradyrhizobium sp.

Organism

Organism UniProt Comment Textmining
Bradyrhizobium sp.
-
-
-
Bradyrhizobium sp. ORS278
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000000083
-
substrate zeaxanthin. 30°C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid Bradyrhizobium sp.
0.00000015
-
substrate beta-carotene, 30°C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid Bradyrhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 2-oxoglutarate + O2
-
Bradyrhizobium sp. canthaxanthin + ?
-
?
beta-carotene + 2-oxoglutarate + O2
-
Bradyrhizobium sp. ORS278 canthaxanthin + ?
-
?
zeaxanthin + 2-oxoglutarate + O2
-
Bradyrhizobium sp. 4-oxozeaxanthin + ?
-
?
zeaxanthin + 2-oxoglutarate + O2
-
Bradyrhizobium sp. ORS278 4-oxozeaxanthin + ?
-
?

Synonyms

Synonyms Comment Organism
CrtW
-
Bradyrhizobium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no activation by NAD*, NADP+, NADPH, FAD, FMN Bradyrhizobium sp.