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Literature summary for 1.14.11.9 extracted from

  • Gebhardt, Y.H.; Witte, S.; Steuber, H.; Matern, U.; Martens, S.
    Evolution of flavone synthase I from parsley flavanone 3beta-hydroxylase by site-directed mutagenesis (2007), Plant Physiol., 144, 1442-1454.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Petroselinum crispum

Protein Variants

Protein Variants Comment Organism
D195E mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol Petroselinum crispum
D195E/L215V/K216R mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol Petroselinum crispum
D331H no catalytic activity Petroselinum crispum
I115T mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol Petroselinum crispum
I115T/V116I mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol Petroselinum crispum
I115T/V116I/I131F/D195E mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 76% flavanone 3beta-hydroxylase product dihydrokaempferol and to 24% flavone synthase product apigenin Petroselinum crispum
I131F mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol Petroselinum crispum
I131F/D195E mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol Petroselinum crispum
I131F/D195E/L215V/K216R mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 69% flavanone 3beta-hydroxylase product dihydrokaempferol and to 31% flavone synthase product apigenin Petroselinum crispum
I131F/L215V/K216R mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 78% flavanone 3beta-hydroxylase product dihydrokaempferol and to 22% flavone synthase product apigenin Petroselinum crispum
L215V/K216R mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol Petroselinum crispum
M106T mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol Petroselinum crispum
M106T/I115T/V116I/I131F/D195E mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 66% flavanone 3beta-hydroxylase product dihydrokaempferol and to 34% flavone synthase product apigenin Petroselinum crispum
M106T/I115T/V116I/I131F/D195E/L215V/K216R mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 18% flavanone 3beta-hydroxylase product dihydrokaempferol and to 82% flavone synthase product apigenin Petroselinum crispum
M106T/I131F/D195E mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 85% flavanone 3beta-hydroxylase product dihydrokaempferol and to 15% flavone synthase product apigenin Petroselinum crispum
additional information domain swapping experiments joining the N-terminus of flavanone 3beta-hydroxylase with the C-terminus of flavone synthase I and vice versa. Active site residues of flavanone 3beta-hydroxylase are M106, I115, V116, I131, D195, V200, L215, and K216 Petroselinum crispum

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-naringenin + 2-oxoglutarate + O2
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 Petroselinum crispum (2R,3R)-dihydrokaempferol + succinate + CO2
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 ?