Literature summary for 1.14.11.9 extracted from

Lukacin, R.; Groning, I.; Pieper, U.; Matern, U.
Site-directed mutagenesis of the active site serine290 in flavanone 3beta-hydroxylase from Petunia hybrida (2000), Eur. J. Biochem., 267, 853-860.

Search for more literature for 1.14.11.9

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant enzymes, expression in Escherichia coli	Petunia x hybrida

Protein Variants

Protein Variants	Comment	Organism
S290A	activity is reduced to 8% of that of the wild-type enzyme	Petunia x hybrida
S290T	activity is reduced to 20% of that of the wild-type enzyme	Petunia x hybrida
S290V	activity is reduced to 1% of that of the wild-type enzyme	Petunia x hybrida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0019	-	2-oxoglutarate	wild-type enzyme	Petunia x hybrida
0.0256	-	2-oxoglutarate	mutant enzyme S290T	Petunia x hybrida
0.08	-	2-oxoglutarate	mutant enzyme S290A	Petunia x hybrida
0.1896	-	2-oxoglutarate	mutant enzyme S290V	Petunia x hybrida

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Petunia x hybrida	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
naringenin + 2-oxoglutarate + O2	Petunia x hybrida	enzyme is involved in the biosynthesis of flavonoids, catechins, and anthocyanidins	(2R,3R)-dihydrokaempferol + succinate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Petunia x hybrida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and mutant enzymes	Petunia x hybrida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
naringenin + 2-oxoglutarate + O2	enzyme is involved in the biosynthesis of flavonoids, catechins, and anthocyanidins	Petunia x hybrida	(2R,3R)-dihydrokaempferol + succinate + CO2	-	?

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Release 2023.1 (January 2023)