Cloned (Comment) | Organism |
---|---|
recombinant expression of the enzyme fused to MBP | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on, required for catalysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 | Homo sapiens | - |
(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NVH6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant MBP-taged enzyme by affinity chromatography, tag cleavage by rTEV protease, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 | - |
Homo sapiens | (3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Nepsilon-trimethyllysine hydroxylase | - |
Homo sapiens |
TMLH | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the family of 2-oxoglutarate (2OG)-dependent oxygenases | Homo sapiens |
metabolism | the enzyme catalyses the first step in mammalian biosynthesis of carnitine, which plays a crucial role in fatty acid metabolism. Carnitine biosynthesis patway, overview | Homo sapiens |