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Literature summary for 1.14.11.57 extracted from

  • Shibasaki, T.; Mori, H.; Chiba, S.; Ozaki, A.
    Microbial proline 4-hydroxylase screening and gene cloning (1999), Appl. Environ. Microbiol., 65, 4028-4031.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.57

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Dactylosporangium sp. RH1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29715
-
-
 Dactylosporangium sp. RH1
31000
-
 gel filtration Dactylosporangium sp. RH1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-proline + 2-oxoglutarate + O2 Dactylosporangium sp. RH1
-
 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Dactylosporangium sp. RH1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Dactylosporangium sp. RH1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2080
-
 pH 6.5, 35°C Dactylosporangium sp. RH1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + 2-oxoglutarate + O2
-
 Dactylosporangium sp. RH1 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE, 1 * 29715, calculated Dactylosporangium sp. RH1