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Literature summary for 1.14.11.57 extracted from

  • Lawrence, C.C.; Sobey, W.J.; Field, R.A.; Baldwin, J.E.; Schofield, C.J.
    Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridis P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis (1996), Biochem. J., 313, 185-191.
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Activating Compound

Activating Compound Comment Organism Structure
catalase presence of catalase causes 20-30% increase in the turnover of L-proline Streptomyces griseoviridis
pyridine-2,6-dicarboxylate
-
 Streptomyces griseoviridis

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate inhibitory above 0.5 mM Streptomyces griseoviridis
3,4-dihydroxybenzoate
-
 Streptomyces griseoviridis
Co2+ 0.5 mM, complete inhibition Streptomyces griseoviridis
diethyl dicarbonate complete inhibition. Inactivation can be partially prevented by the inclusion of L-proline and 2-oxoglutarate in the preincubation mixture Streptomyces griseoviridis
L-ascorbate ascorbate causes a time-dependent inhibition of L-proline hydroxylation. The addition of ascorbate does not stimulate L-proline-coupled turnover of 2-oxoglutarate, but does stimulate L-proline-uncoupled turnover Streptomyces griseoviridis
Pyridine-2,4-dicarboxylate competitive with respect to 2-oxoglutarate Streptomyces griseoviridis
Pyridine-2,5-dicarboxylate
-
 Streptomyces griseoviridis
Zn2+ 0.5 mM, complete inhibition Streptomyces griseoviridis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 2-oxoglutarate pH 7.5, 35°C Streptomyces griseoviridis
0.445
-
 L-proline pH 7.5, 35°C Streptomyces griseoviridis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ about 10-15% of the activity with iron Streptomyces griseoviridis
Iron strict requirements for ferrous ion and 2-oxoglutarate, maximum activity at 0.03 mM Streptomyces griseoviridis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 1 * 35000, SDS-PAGE Streptomyces griseoviridis
38000
-
 gel filtration Streptomyces griseoviridis

Organism

Organism UniProt Comment Textmining
Streptomyces griseoviridis
-
-
-
Streptomyces griseoviridis P8648
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
907
-
 pH 7.5, 26°C Streptomyces griseoviridis

Storage Stability

Storage Stability Organism
-20°C, about 30% loss of activity by freezing and thawing Streptomyces griseoviridis
4°C, 4-5 h, stable Streptomyces griseoviridis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + 2-oxoglutarate + O2
-
 Streptomyces griseoviridis trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Streptomyces griseoviridis P8648 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
additional information the enzyme displays strict requirements for ferrous ion and 2-oxoglutarate, no cosubstrate: 2-oxopentanoate, 2-oxoadipate, pyruvate or 2-oxomalonate. L-Proline-uncoupled turnover of 2-oxoglutarate to succinate and CO2 is observed Streptomyces griseoviridis ?
-
 ?
additional information the enzyme displays strict requirements for ferrous ion and 2-oxoglutarate, no cosubstrate: 2-oxopentanoate, 2-oxoadipate, pyruvate or 2-oxomalonate. L-Proline-uncoupled turnover of 2-oxoglutarate to succinate and CO2 is observed Streptomyces griseoviridis P8648 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Streptomyces griseoviridis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.005
-
 pH 7.5, 35°C Streptomyces griseoviridis Pyridine-2,4-dicarboxylate
0.032
-
 pH 7.5, 35°C Streptomyces griseoviridis 3,4-dihydroxybenzoate
0.049
-
 pH 7.5, 35°C Streptomyces griseoviridis Pyridine-2,5-dicarboxylate