Literature summary for 1.14.11.55 extracted from

Widderich, N.; Hoeppner, A.; Pittelkow, M.; Heider, J.; Smits, S.H.; Bremer, E.
Biochemical properties of ectoine hydroxylases from extremophiles and their wider taxonomic distribution among microorganisms (2014), PLoS ONE, 9, e93809.

Search for more literature for 1.14.11.55

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 1.9 A resolution, and comparison of iron-bound and apo structure. The iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/EH motif, the so-called 2-His-1-carboxylate facial triad	Virgibacillus salexigens

Inhibitors

Inhibitors	Comment	Organism
NaCl	maximum activity in presence of 50 mM KCl. High concentrations of NaCl are inhibitory	Acidiphilium cryptum
NaCl	maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory	Alkalilimnicola ehrlichii
NaCl	maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory	Halomonas elongata
NaCl	maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory	Paenibacillus lautus
NaCl	maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory	Pseudomonas stutzeri
NaCl	maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory	Sphingopyxis alaskensis
NaCl	maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory	Virgibacillus salexigens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
2.7	-	2-oxoglutarate	pH 7.5, 32°C	Sphingopyxis alaskensis
3.9	-	2-oxoglutarate	pH 7.5, 32°C	Paenibacillus lautus
4.1	-	2-oxoglutarate	pH 7.5, 32°C	Acidiphilium cryptum
4.6	-	2-oxoglutarate	pH 7.5, 32°C	Pseudomonas stutzeri
4.8	-	2-oxoglutarate	pH 7.5, 32°C	Halomonas elongata
4.9	-	2-oxoglutarate	pH 7.5, 32°C	Virgibacillus salexigens
5	-	2-oxoglutarate	pH 7.5, 32°C	Alkalilimnicola ehrlichii
5.7	-	ectoine	pH 7.5, 32°C	Halomonas elongata
5.9	-	ectoine	pH 7.5, 32°C	Virgibacillus salexigens
6.2	-	ectoine	pH 7.5, 32°C	Pseudomonas stutzeri
9	-	ectoine	pH 7.5, 32°C	Alkalilimnicola ehrlichii
9.5	-	ectoine	pH 7.5, 32°C	Paenibacillus lautus
9.8	-	ectoine	pH 7.5, 32°C	Sphingopyxis alaskensis
10	-	ectoine	pH 7.5, 32°C	Acidiphilium cryptum

Metals/Ions

Metals/Ions	Comment	Organism
Iron	the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/EH motif, the so-called 2-His-1-carboxylate facial triad	Pseudomonas stutzeri
Iron	the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/EH motif, the so-called 2-His-1-carboxylate facial triad	Virgibacillus salexigens
Iron	the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/EH motif, the so-called 2-His-1-carboxylate facial triad	Sphingopyxis alaskensis
Iron	the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/EH motif, the so-called 2-His-1-carboxylate facial triad	Halomonas elongata
Iron	the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/EH motif, the so-called 2-His-1-carboxylate facial triad	Paenibacillus lautus
Iron	the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/EH motif, the so-called 2-His-1-carboxylate facial triad	Alkalilimnicola ehrlichii
Iron	the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/EH motif, the so-called 2-His-1-carboxylate facial triad	Acidiphilium cryptum
KCl	maximum activity in presence of 100 mM KCl	Sphingopyxis alaskensis
KCl	maximum activity in presence of 100 mM KCl	Acidiphilium cryptum
KCl	maximum activity in presence of 150 mM KCl	Pseudomonas stutzeri
KCl	maximum activity in presence of 150 mM KCl	Virgibacillus salexigens
KCl	maximum activity in presence of 150 mM KCl	Halomonas elongata
KCl	maximum activity in presence of 150 mM KCl	Alkalilimnicola ehrlichii
KCl	maximum activity in presence of 200 mM KCl	Paenibacillus lautus
NaCl	maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory	Pseudomonas stutzeri
NaCl	maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory	Virgibacillus salexigens
NaCl	maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory	Sphingopyxis alaskensis
NaCl	maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory	Halomonas elongata
NaCl	maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory	Paenibacillus lautus
NaCl	maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory	Alkalilimnicola ehrlichii
NaCl	maximum activity in presence of 50 mM KCl. High concentrations of NaCl are inhibitory	Acidiphilium cryptum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34100	-	-	Sphingopyxis alaskensis
34100	-	-	Acidiphilium cryptum
34200	-	x * 34200, SDS-PAGE	Pseudomonas stutzeri
34300	-	x * 34300, SDS-PAGE	Alkalilimnicola ehrlichii
34400	-	x * 34400, SDS-PAGE	Virgibacillus salexigens
34800	-	x * 34800, SDS-PAGE	Paenibacillus lautus
37400	-	-	Halomonas elongata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ectoine + 2-oxoglutarate + O2	Pseudomonas stutzeri	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Virgibacillus salexigens	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Sphingopyxis alaskensis	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Halomonas elongata	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Paenibacillus lautus	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Alkalilimnicola ehrlichii	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Acidiphilium cryptum	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Pseudomonas stutzeri A1501	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Sphingopyxis alaskensis DSM 13593	-	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	Halomonas elongata DSM 2581	-	5-hydroxyectoine + succinate + CO2	-	ir
additional information	Pseudomonas stutzeri	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Virgibacillus salexigens	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Sphingopyxis alaskensis	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Halomonas elongata	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Paenibacillus lautus	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Alkalilimnicola ehrlichii	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Acidiphilium cryptum	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Pseudomonas stutzeri A1501	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Sphingopyxis alaskensis DSM 13593	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?
additional information	Halomonas elongata DSM 2581	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Acidiphilium cryptum	JN019030	ectD gene optimized for the expression in Escherichia coli	-
Alkalilimnicola ehrlichii	JN019031	ectD gene optimized for the expression in Escherichia coli	-
Halomonas elongata	E1VA04	-	-
Halomonas elongata DSM 2581	E1VA04	-	-
Paenibacillus lautus	A0A1R1AUM9	ectD gene optimized for the expression in Escherichia coli	-
Pseudomonas stutzeri	-	-	-
Pseudomonas stutzeri A1501	-	-	-
Sphingopyxis alaskensis	Q1GNW5	-	-
Sphingopyxis alaskensis DSM 13593	Q1GNW5	-	-
Virgibacillus salexigens	Q2TDY4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ectoine + 2-oxoglutarate + O2	-	Pseudomonas stutzeri	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Virgibacillus salexigens	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Sphingopyxis alaskensis	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Halomonas elongata	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Paenibacillus lautus	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Alkalilimnicola ehrlichii	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Acidiphilium cryptum	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Pseudomonas stutzeri A1501	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Sphingopyxis alaskensis DSM 13593	5-hydroxyectoine + succinate + CO2	-	ir
ectoine + 2-oxoglutarate + O2	-	Halomonas elongata DSM 2581	5-hydroxyectoine + succinate + CO2	-	ir
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Pseudomonas stutzeri	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Virgibacillus salexigens	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Sphingopyxis alaskensis	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Halomonas elongata	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Paenibacillus lautus	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Alkalilimnicola ehrlichii	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Acidiphilium cryptum	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Pseudomonas stutzeri A1501	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Sphingopyxis alaskensis DSM 13593	?	-	?
additional information	ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine	Halomonas elongata DSM 2581	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 34200, SDS-PAGE	Pseudomonas stutzeri
?	x * 34400, SDS-PAGE	Virgibacillus salexigens
?	x * 34800, SDS-PAGE	Paenibacillus lautus
?	x * 34300, SDS-PAGE	Alkalilimnicola ehrlichii
?	x * 34100, SDS-PAGE	Sphingopyxis alaskensis
?	x * 34100, SDS-PAGE	Acidiphilium cryptum
?	x * 37400, SDS-PAGE	Halomonas elongata

Synonyms

Synonyms	Comment	Organism
ectD	-	Pseudomonas stutzeri
ectD	-	Virgibacillus salexigens
ectD	-	Sphingopyxis alaskensis
ectD	-	Halomonas elongata
ectD	-	Paenibacillus lautus
ectD	-	Alkalilimnicola ehrlichii
ectD	-	Acidiphilium cryptum
Sala_2952	-	Sphingopyxis alaskensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
32	-	-	Virgibacillus salexigens
32	-	-	Halomonas elongata
32	-	-	Acidiphilium cryptum
35	-	-	Pseudomonas stutzeri
35	-	-	Alkalilimnicola ehrlichii
40	-	-	Sphingopyxis alaskensis
40	-	-	Paenibacillus lautus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
5	50	-	Virgibacillus salexigens
5	50	-	Sphingopyxis alaskensis
5	47	-	Halomonas elongata
10	50	-	Pseudomonas stutzeri
10	47	-	Acidiphilium cryptum
15	50	-	Paenibacillus lautus
15	45	-	Alkalilimnicola ehrlichii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
1.2	-	ectoine	pH 7.5, 32°C	Sphingopyxis alaskensis
1.2	-	ectoine	pH 7.5, 32°C	Alkalilimnicola ehrlichii
1.6	-	ectoine	pH 7.5, 32°C	Paenibacillus lautus
2.8	-	ectoine	pH 7.5, 32°C	Halomonas elongata
3.4	-	ectoine	pH 7.5, 32°C	Acidiphilium cryptum
7.7	-	ectoine	pH 7.5, 32°C	Virgibacillus salexigens
8.9	-	ectoine	pH 7.5, 32°C	Pseudomonas stutzeri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Pseudomonas stutzeri
7.5	-	-	Virgibacillus salexigens
7.5	-	-	Paenibacillus lautus
7.5	-	-	Alkalilimnicola ehrlichii
8	-	-	Sphingopyxis alaskensis
8	-	-	Halomonas elongata
8	-	-	Acidiphilium cryptum

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	9.6	-	Pseudomonas stutzeri
5.5	9.6	-	Virgibacillus salexigens
5.5	9.6	-	Sphingopyxis alaskensis
5.5	9.6	-	Paenibacillus lautus
5.5	9.6	-	Acidiphilium cryptum
6.5	9.6	-	Halomonas elongata
6.5	9.6	-	Alkalilimnicola ehrlichii

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pseudomonas stutzeri	calculated	-	5.5
Sphingopyxis alaskensis	calculated	-	5.5
Paenibacillus lautus	calculated	-	5.6
Alkalilimnicola ehrlichii	calculated	-	5.7
Virgibacillus salexigens	calculated	-	5.8
Halomonas elongata	calculated	-	5.8
Acidiphilium cryptum	calculated	-	5.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.12	-	ectoine	pH 7.5, 32°C	Sphingopyxis alaskensis
0.13	-	ectoine	pH 7.5, 32°C	Alkalilimnicola ehrlichii
0.17	-	ectoine	pH 7.5, 32°C	Paenibacillus lautus
0.34	-	ectoine	pH 7.5, 32°C	Acidiphilium cryptum
0.49	-	ectoine	pH 7.5, 32°C	Halomonas elongata
1.31	-	ectoine	pH 7.5, 32°C	Virgibacillus salexigens
1.44	-	ectoine	pH 7.5, 32°C	Pseudomonas stutzeri