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Literature summary for 1.14.11.55 extracted from

  • Hoeppner, A.; Widderich, N.; Lenders, M.; Bremer, E.; Smits, S.H.
    Crystal structure of the ectoine hydroxylase, a snapshot of the active site (2014), J. Biol. Chem., 289, 29570-29583.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure in its apo-form, in complex with iron, and in complex with iron, cosubstrate 2-oxoglutarate, and 5-hydroxyectoine. The iron and 2-oxoglutarate ligands are bound within the active site in a fashion similar to that found in other members of the dioxygenase superfamily. 5-Hydroxyectoine is bound by residues residues His144, His245, and Asp146 forming the 2-His-1-carboxylate facial triad Sphingopyxis alaskensis

Protein Variants

Protein Variants Comment Organism
E140A residue involved in dimerization, activity similar to wild-type Sphingopyxis alaskensis
Q127A residue involved in ectoine binding, about 1% of wild-type activity Sphingopyxis alaskensis
R139A residue involved in dimerization, activity similar to wild-type Sphingopyxis alaskensis
R139A/E140A residues involved in dimerization, activity similar to wild-type Sphingopyxis alaskensis
R280A residue involved in ectoine binding, about 8% of wild-type activity Sphingopyxis alaskensis
T149A residue involved in ectoine binding, about 4% of wild-type activity Sphingopyxis alaskensis
W150A residue involved in ectoine binding, about 2% of wild-type activity Sphingopyxis alaskensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
2-oxoglutarate pH 8.0, 40°C Sphingopyxis alaskensis
9.8
-
ectoine pH 8.0, 40°C Sphingopyxis alaskensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35290
-
-
Sphingopyxis alaskensis
70730
-
light scattering, recombinant Strep-tagged protein Sphingopyxis alaskensis

Organism

Organism UniProt Comment Textmining
Sphingopyxis alaskensis Q1GNW5
-
-
Sphingopyxis alaskensis DSM 13593 Q1GNW5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ectoine + 2-oxoglutarate + O2
-
Sphingopyxis alaskensis 5-hydroxyectoine + succinate + CO2
-
?
ectoine + 2-oxoglutarate + O2
-
Sphingopyxis alaskensis DSM 13593 5-hydroxyectoine + succinate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35290, calculated, recombinant Strep-tagged protein Sphingopyxis alaskensis

Synonyms

Synonyms Comment Organism
Sala_2952
-
Sphingopyxis alaskensis