Crystallization (Comment) | Organism |
---|---|
crystal structure in its apo-form, in complex with iron, and in complex with iron, cosubstrate 2-oxoglutarate, and 5-hydroxyectoine. The iron and 2-oxoglutarate ligands are bound within the active site in a fashion similar to that found in other members of the dioxygenase superfamily. 5-Hydroxyectoine is bound by residues residues His144, His245, and Asp146 forming the 2-His-1-carboxylate facial triad | Sphingopyxis alaskensis |
Protein Variants | Comment | Organism |
---|---|---|
E140A | residue involved in dimerization, activity similar to wild-type | Sphingopyxis alaskensis |
Q127A | residue involved in ectoine binding, about 1% of wild-type activity | Sphingopyxis alaskensis |
R139A | residue involved in dimerization, activity similar to wild-type | Sphingopyxis alaskensis |
R139A/E140A | residues involved in dimerization, activity similar to wild-type | Sphingopyxis alaskensis |
R280A | residue involved in ectoine binding, about 8% of wild-type activity | Sphingopyxis alaskensis |
T149A | residue involved in ectoine binding, about 4% of wild-type activity | Sphingopyxis alaskensis |
W150A | residue involved in ectoine binding, about 2% of wild-type activity | Sphingopyxis alaskensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
2-oxoglutarate | pH 8.0, 40°C | Sphingopyxis alaskensis | |
9.8 | - |
ectoine | pH 8.0, 40°C | Sphingopyxis alaskensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35290 | - |
- |
Sphingopyxis alaskensis |
70730 | - |
light scattering, recombinant Strep-tagged protein | Sphingopyxis alaskensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingopyxis alaskensis | Q1GNW5 | - |
- |
Sphingopyxis alaskensis DSM 13593 | Q1GNW5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ectoine + 2-oxoglutarate + O2 | - |
Sphingopyxis alaskensis | 5-hydroxyectoine + succinate + CO2 | - |
? | |
ectoine + 2-oxoglutarate + O2 | - |
Sphingopyxis alaskensis DSM 13593 | 5-hydroxyectoine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35290, calculated, recombinant Strep-tagged protein | Sphingopyxis alaskensis |
Synonyms | Comment | Organism |
---|---|---|
Sala_2952 | - |
Sphingopyxis alaskensis |