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Literature summary for 1.14.11.53 extracted from

  • Li, F.; Kennedy, S.; Hajian, T.; Gibson, E.; Seitova, A.; Xu, C.; Arrowsmith, C.H.; Vedadi, M.
    A Radioactivity-based assay for screening human m6A-RNA methyltransferase, METTL3-METTL14 complex, and demethylase ALKBH5 (2016), J. Biomol. Screen., 21, 290-297.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
Homo sapiens
IOX1 competitive inhibition Homo sapiens
additional information the presence of S-adenosyl-L-homocysteine, NaCl, MgCl2, dimethylsulfoxide, and Triton X-100 does not influence enzyme activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000192
-
5'-UACACUCGAUCUGG(m6A)CUAAAGCUGCUC-3'-biotin at pH 8.0 and 37°C Homo sapiens
0.0025
-
2-oxoglutarate at pH 8.0 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on, 0.02 mM Fe2+ used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-methyladenine in mRNA + 2-oxoglutarate + O2 Homo sapiens
-
adenine in mRNA + formaldehyde + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DE52 column chromatography, Ni-NTA column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-UACACUCGAUCUGG(m6A)CUAAAGCUGCUC-3'-biotin + 2-oxoglutarate + O2
-
Homo sapiens 5'-UACACUCGAUCUGGCUAAAGCUGCUC-3'-biotin + formaldehyde + succinate + CO2
-
?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
-
Homo sapiens adenine in mRNA + formaldehyde + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
ALKBH5
-
Homo sapiens
m6A-RNA demethylase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
2-oxoglutarate at pH 8.0 and 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
120
-
2-oxoglutarate at pH 8.0 and 37°C Homo sapiens