Application | Comment | Organism |
---|---|---|
synthesis | double mutant I162T/T182N shows improvements in specific activity, protein expression level, and fermentation titer of 3.2-, 2.8-, and 9.4fold, respectively. L-Isoleucine (228 mM) is completely converted to (2S,3R,4S)-4-HIL with a space-time yield of up to 80.8 g/l and d. With a increase of the substrate loading to 1 M, a high conversion of 91% can also be achieved | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
I162T | specific activity is 1.9fold higher than that of wild-type | Bacillus subtilis |
I162T/T182N | double mutant with improvements in specific activity, protein expression level, and fermentation titer of 3.2-, 2.8-, and 9.4fold, respectively | Bacillus subtilis |
T182N | mutant achieves up to 70% conversion of Ile | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
mutant T182N, pH 7.0, 30°C | Bacillus subtilis |
3.8 | - |
wild-type, pH 7.0, 30°C | Bacillus subtilis |
6.4 | - |
mutant I162T, pH 7.0, 30°C | Bacillus subtilis |
12 | - |
mutant I162T/T182N, pH 7.0, 30°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus subtilis | (4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus subtilis |