Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | slightly reduced in the absence of ascorbate | Sphingobium herbicidovorans |
Cloned (Comment) | Organism |
---|---|
expressed as His6-tagged fusion protein from Escherichia coli BL21(DE3)(pLysS) | Sphingobium herbicidovorans |
General Stability | Organism |
---|---|
storage on ice and freeze-thaw cycles reduce the activity to about 80% of the original activity | Sphingobium herbicidovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-2-(2,4-dichlorophenoxy)propanoate | slight inhibition | Sphingobium herbicidovorans | |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | slight inhibition | Sphingobium herbicidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0196 | - |
2-oxoglutarate | pH 6.8, 30°C | Sphingobium herbicidovorans | |
0.132 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C | Sphingobium herbicidovorans | |
0.159 | - |
O2 | pH 6.8, 30°C, above 0.23 mM | Sphingobium herbicidovorans | |
0.495 | - |
(S)-(2,4-dichlorophenoxy)propanoate | pH 6.8, 30°C | Sphingobium herbicidovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | ferrous iron is necessary for activity of the enzyme and other divalent cations could not replace it | Sphingobium herbicidovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration | Sphingobium herbicidovorans |
33700 | - |
1 * 33700, calculated from sequence | Sphingobium herbicidovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 | Sphingobium herbicidovorans | i.e. (S)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate | 2,4-dichlorophenol + pyruvate + succinate + CO2 | - |
? | |
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 | Sphingobium herbicidovorans | i.e. (S)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate | 2-methyl-4-chlorophenol + pyruvate + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium herbicidovorans | Q700X4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium herbicidovorans |
Storage Stability | Organism |
---|---|
4°C, 3 days, less than 10% loss of activity | Sphingobium herbicidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 | i.e. (S)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate | Sphingobium herbicidovorans | 2,4-dichlorophenol + pyruvate + succinate + CO2 | - |
? | |
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 | i.e. (S)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate | Sphingobium herbicidovorans | 2-methyl-4-chlorophenol + pyruvate + succinate + CO2 | - |
? | |
(S)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 | i.e. (S)-dichlorprop. SdpA is specific for the (S) enantiomer | Sphingobium herbicidovorans | 2,4-dichlorophenol + pyruvate + succinate + CO2 | - |
? | |
(S)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 | i.e. (S)-mecoprop. SdpA is specific for the (S) enantiomer. The enzyme is specific for 2-oxoglutarate. Low levels of activity (1.9%) in presence of 2-oxoadipate. No activity with oxaloacetate, 2-oxobutyrate, and 2-oxovalerate | Sphingobium herbicidovorans | 4-chloro-2-methylphenol + pyruvate + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE | Sphingobium herbicidovorans |
monomer | 1 * 33700, calculated from sequence | Sphingobium herbicidovorans |
Synonyms | Comment | Organism |
---|---|---|
2-oxoglutarate-dependent (S)-dichlorprop dioxygenase | - |
Sphingobium herbicidovorans |
alpha-ketoglutarate-dependent (S)-dichlorprop dioxygenase | - |
Sphingobium herbicidovorans |
SdpA | - |
Sphingobium herbicidovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Sphingobium herbicidovorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | 20°C: about 40% of maximal activity, 35-40°C: optimal activity | Sphingobium herbicidovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
stable for several hours | Sphingobium herbicidovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Sphingobium herbicidovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | pH 6.0: about 30% of maximal activity, pH 7.5: about 50% of maximal activity | Sphingobium herbicidovorans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH | Sphingobium herbicidovorans |