Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Streptomyces vinaceus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor-diffusion method. The crystal structures of VioC are determined in complexes with the cofactor Fe(II), the substrate L-arginine, the product (2S,3S)-hydroxyarginine and the coproduct succinate at 1.11.3 A resolution | Streptomyces vinaceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.16 | - |
L-canavanine | pH 8.0, 30°C | Streptomyces vinaceus | |
3.4 | - |
L-arginine | pH 8.0, 30°C | Streptomyces vinaceus | |
7.05 | - |
L-homoarginine | pH 8.0, 30°C | Streptomyces vinaceus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+-dependent enzyme | Streptomyces vinaceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41600 | - |
x * 41600, SDS-PAGE | Streptomyces vinaceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces vinaceus | Q6WZB0 | - |
- |
Purification (Comment) | Organism |
---|---|
expressed as a fusion protein with an N-terminal hexahistidine tag in Escherichia coli BL21(DE3) | Streptomyces vinaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + 2-oxoglutarate + O2 | L-arginine is the preferred substrate | Streptomyces vinaceus | (3S)-3-hydroxy-L-arginine + succinate + CO2 | - |
? | |
L-canavanine + 2-oxoglutarate + O2 | - |
Streptomyces vinaceus | ? + succinate + CO2 | - |
? | |
L-homoarginine + 2-oxoglutarate + O2 | - |
Streptomyces vinaceus | (3S)-N6-(diaminomethylidene)-3-hydroxy-L-lysine + succinate + CO2 | - |
? | |
additional information | no activity with D-arginine, NG-methyl-L-arginine, NG-hydroxynor-L-arginine | Streptomyces vinaceus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41600, SDS-PAGE | Streptomyces vinaceus |
Synonyms | Comment | Organism |
---|---|---|
VioC | - |
Streptomyces vinaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces vinaceus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.22 | - |
L-canavanine | pH 8.0, 30°C | Streptomyces vinaceus | |
13.85 | - |
L-homoarginine | pH 8.0, 30°C | Streptomyces vinaceus | |
43.5 | - |
L-arginine | pH 8.0, 30°C | Streptomyces vinaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces vinaceus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the tuberactinomycin antibiotic viomycin | Streptomyces vinaceus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
L-canavanine | pH 8.0, 30°C | Streptomyces vinaceus | |
1.96 | - |
L-homoarginine | pH 8.0, 30°C | Streptomyces vinaceus | |
12.8 | - |
L-arginine | pH 8.0, 30°C | Streptomyces vinaceus |