Cloned (Comment) | Organism |
---|---|
all 3 isozymes LH1-3, LH2 in 2 splicing variants, are expressed in as His-tagged proteins in Spodoptera frugiperda Sf9 cells via baculovirus infection system with the signal peptide at the N-terminus in isozymes LH2a and b and LH3 being exchanged for the signal peptide of isozyme LH1 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | reduces the activity of the isozymes LH1, LH2a, LH2b, and LH3 with most of the synthetic nonpeptide substrates tested, in some cases the isozyme is activated or completely inhibited, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km of recombinant isozymes LH1, LH2a, LH2b, and LH3 for diverse synthetic nonpeptide substrates, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | reduces the activity of the isozymes LH1, LH2a, LH2b, and LH3 with most of the synthetic nonpeptide substrates tested, in some cases the isozyme is activated or completely inhibited, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-lysine + 2-oxoglutarate + O2 | Homo sapiens | - |
procollagen 5-hydroxy-L-lysine + succinate + CO2 | - |
? | |
procollagen L-lysine + 2-oxoglutarate + O2 | Caenorhabditis elegans | collagen and other proteins with collagenous domains | procollagen 5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
1 enzyme form LH, no isozymes | - |
Homo sapiens | - |
3 isozymes LH1-3, isozyme LH2 exists in 2 splicing variants LH2a and LH2b | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes LH1-3 from Sf9 insect cells by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine containing nonapeptides + 2-oxoglutarate + O2 | diverse nonapeptides, synthetic substrates | Homo sapiens | 5-hydroxy-L-lysine containing nonapeptides + succinate + CO2 | - |
? | |
additional information | isozymes show no strict substrate specificity concerning the synthetic peptide substrates, but preferences for sequences, analysis of the different isozymes' binding affinities for the peptides whose net charges are very important, partly the peptides bind to the active site, overview | Homo sapiens | ? | - |
? | |
additional information | the enzyme is also able to glycosylate hydroxylysyl residues and is functionally similar to the isozyme LH3 of vertebrates | Caenorhabditis elegans | ? | - |
? | |
procollagen L-lysine + 2-oxoglutarate + O2 | - |
Homo sapiens | procollagen 5-hydroxy-L-lysine + succinate + CO2 | - |
? | |
procollagen L-lysine + 2-oxoglutarate + O2 | collagen and other proteins with collagenous domains | Caenorhabditis elegans | procollagen 5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LH | - |
Homo sapiens |
LH | - |
Caenorhabditis elegans |
lysyl hydroxylase | - |
Homo sapiens |
lysyl hydroxylase | - |
Caenorhabditis elegans |