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Literature summary for 1.14.11.35 extracted from

  • You, Z.; Omura, S.; Ikeda, H.; Cane, D.E.
    Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis (2006), J. Am. Chem. Soc., 128, 6566-6567.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces avermitilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
(+/-)-1-deoxypentalenic acid pH 6.0, temperature not specified in the publication Streptomyces avermitilis

Metals/Ions

Metals/Ions Comment Organism Structure
Iron non-heme iron, alpha-ketoglutarate-dependent hydroxylase Streptomyces avermitilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37121
-
x * 37121, calculated, x * 37139, MALDI-TOF Streptomyces avermitilis
37139
-
x * 37121, calculated, x * 37139, MALDI-TOF Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q82IZ1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-1-deoxypentalenic acid + O2
-
Streptomyces avermitilis 11beta-hydroxy-1-deoxypentalenic acid + succinate + CO2
-
?
additional information no substrate: (+/-)-pentalenene, (+/-)-pentalen-13-ol Streptomyces avermitilis ?
-
?

Subunits

Subunits Comment Organism
? x * 37121, calculated, x * 37139, MALDI-TOF Streptomyces avermitilis

Synonyms

Synonyms Comment Organism
ptlH
-
Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
(+/-)-1-deoxypentalenic acid pH 6.0, temperature not specified in the publication Streptomyces avermitilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Streptomyces avermitilis