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Literature summary for 1.14.11.33 extracted from

  • Li, D.; Delaney, J.C.; Page, C.M.; Yang, X.; Chen, A.S.; Wong, C.; Drennan, C.L.; Essigmann, J.M.
    Exocyclic carbons adjacent to the N6 of adenine are targets for oxidation by the Escherichia coli adaptive response protein AlkB (2012), J. Am. Chem. Soc., 134, 8896-8901.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli exocyclic carbons adjacent to the N6 of adenine are targets for oxidation by the Escherichia coli adaptive response protein AlkB ?
-
?
N1-methyladenine + 2-oxoglutarate + O2 Escherichia coli
-
adenine + formaldehyde + succinate + CO2
-
?
N6-methyladenine + 2-oxoglutarate + O2 Escherichia coli via 6-hydroxymethyl adenine derivative adenine + formaldehyde + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information exocyclic carbons adjacent to the N6 of adenine are targets for oxidation by the Escherichia coli adaptive response protein AlkB Escherichia coli ?
-
?
N1-methyladenine + 2-oxoglutarate + O2
-
Escherichia coli adenine + formaldehyde + succinate + CO2
-
?
N6-methyladenine + 2-oxoglutarate + O2 via 6-hydroxymethyl adenine derivative Escherichia coli adenine + formaldehyde + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
AlkB
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli

General Information

General Information Comment Organism
physiological function the DNA and RNA repair protein AlkB removes alkyl groups from nucleic acids by a unique iron- and 2-oxoglutarate-dependent oxidation strategy, role for AlkB in regulation of important cellular functions in vivo. Complete adduct restructuring of ethano-adenine, a DNA adduct formed by an important anticancer drug, BCNU, by AlkB to a form that does not hinder replication. Ethano-adenine dealkylation process requires two oxidation reactions, one at N1 and a second at N6, to achieve complete restoration of the undamaged adenine base. AlkB DNA substrate N6-methyladenine is an important epigenetic signal for DNA replication and many other cellular processes Escherichia coli