Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | exocyclic carbons adjacent to the N6 of adenine are targets for oxidation by the Escherichia coli adaptive response protein AlkB | ? | - |
? | |
N1-methyladenine + 2-oxoglutarate + O2 | Escherichia coli | - |
adenine + formaldehyde + succinate + CO2 | - |
? | |
N6-methyladenine + 2-oxoglutarate + O2 | Escherichia coli | via 6-hydroxymethyl adenine derivative | adenine + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | exocyclic carbons adjacent to the N6 of adenine are targets for oxidation by the Escherichia coli adaptive response protein AlkB | Escherichia coli | ? | - |
? | |
N1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | adenine + formaldehyde + succinate + CO2 | - |
? | |
N6-methyladenine + 2-oxoglutarate + O2 | via 6-hydroxymethyl adenine derivative | Escherichia coli | adenine + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the DNA and RNA repair protein AlkB removes alkyl groups from nucleic acids by a unique iron- and 2-oxoglutarate-dependent oxidation strategy, role for AlkB in regulation of important cellular functions in vivo. Complete adduct restructuring of ethano-adenine, a DNA adduct formed by an important anticancer drug, BCNU, by AlkB to a form that does not hinder replication. Ethano-adenine dealkylation process requires two oxidation reactions, one at N1 and a second at N6, to achieve complete restoration of the undamaged adenine base. AlkB DNA substrate N6-methyladenine is an important epigenetic signal for DNA replication and many other cellular processes | Escherichia coli |