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Literature summary for 1.14.11.33 extracted from

  • Vagbo, C.B.; Svaasand, E.K.; Aas, P.A.; Krokan, H.E.
    Methylation damage to RNA induced in vivo in Escherichia coli is repaired by endogenous AlkB as part of the adaptive response (2013), DNA Repair, 12, 188-195.
    View publication on PubMed
Search for more literature for 1.14.11.33

Cloned(Commentary)

Cloned (Comment) Organism
AlkB overexpression in Escherichia coli AlkB-deficient strains HK82 and BW25113. AlkB mutant strain HK82 is transformed with the low-copy-number expression plasmid pJB658 containing the alkB gene following a toluic acid-inducible promoter. The recombinant AlkB expressed is less active than the natural endogenous form Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli
-
 DNA-base + formaldehyde + succinate + CO2
-
 ?
DNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli AB1157
-
 DNA-base + formaldehyde + succinate + CO2
-
 ?
N1-methyladenine + 2-oxoglutarate + O2 Escherichia coli
-
 adenine + formaldehyde + succinate + CO2
-
 ?
N1-methyladenine + 2-oxoglutarate + O2 Escherichia coli AB1157
-
 adenine + formaldehyde + succinate + CO2
-
 ?
N3-methylcytosine + 2-oxoglutarate + O2 Escherichia coli
-
 cytosine + formaldehyde + succinate + CO2
-
 ?
N3-methylcytosine + 2-oxoglutarate + O2 Escherichia coli AB1157
-
 cytosine + formaldehyde + succinate + CO2
-
 ?
RNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli
-
 RNA-base + formaldehyde + succinate + CO2
-
 ?
RNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli AB1157
-
 RNA-base + formaldehyde + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli AB1157
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli DNA-base + formaldehyde + succinate + CO2
-
 ?
DNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli AB1157 DNA-base + formaldehyde + succinate + CO2
-
 ?
N1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli adenine + formaldehyde + succinate + CO2
-
 ?
N1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli AB1157 adenine + formaldehyde + succinate + CO2
-
 ?
N3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli cytosine + formaldehyde + succinate + CO2
-
 ?
N3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli AB1157 cytosine + formaldehyde + succinate + CO2
-
 ?
RNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli RNA-base + formaldehyde + succinate + CO2
-
 ?
RNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli AB1157 RNA-base + formaldehyde + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
AlkB
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli

General Information

General Information Comment Organism
malfunction an AlkB-deficient strain accumulates N1-methyladenine at a high rate Escherichia coli
additional information the recombinant AlkB expressed is less active than the natural endogenous form Escherichia coli
physiological function protein AlkB repairs cytotoxic 1-methyladenine and 3-methylcytosine lesions induced by methyl methanesulfonate in DNA and RNA in vitro and in pre-damaged DNA and RNA bacteriophages in vivo, quantification of AlkB-mediated repair of endogenous RNA and DNA in vivo, overview Escherichia coli