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Literature summary for 1.14.11.33 extracted from

  • Mishina, Y.; Lee, C.H.; He, C.
    Interaction of human and bacterial AlkB proteins with DNA as probed through chemical cross-linking studies (2004), Nucleic Acids Res., 32, 1548-1554.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H131C the mutant slightly prefers single-stranded DNA substrates over any double-stranded DNA Homo sapiens
H171C the ABH2 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins and cross-links efficiently with the single-stranded DNA-1 and DNA3-6 Homo sapiens
H191C the ABH3 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins and cross-links to DNA-1 much more efficiently than the wild type protein after a 20 h incubation Homo sapiens
H231C the ABH1 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins, no cross-link between the mutant protein and the single-stranded and double-stranded DNA probes is observed Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Escherichia coli
Fe2+ required for activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, S-Sepharose column chromatography, Ni-NTA column chromatography, and Mono-S cation exchange column chromatography Escherichia coli
DEAE-cellulose column chromatography, S-Sepharose column chromatography, Ni-NTA column chromatography, and Mono-S cation exchange column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-1-methyladenine + 2-oxoglutarate + O2
-
Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
?
DNA-1-methyladenine + 2-oxoglutarate + O2
-
Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
?
DNA-3-methylcytosine + 2-oxoglutarate + O2
-
Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
?
DNA-3-methylcytosine + 2-oxoglutarate + O2
-
Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
?
additional information ABH3 prefers single-stranded DNA and RNA substrates, whereas ABH2 can repair damage in both single- and double-stranded DNA. Isoform ABH1 does not show any DNA repair activity Homo sapiens ?
-
?
additional information AlkB can repair damage in both single- and double-stranded DNA Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
ABH1 isoform Homo sapiens
ABH2 isoform Homo sapiens
ABH3 isoform Homo sapiens
AlkB
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
Escherichia coli
2-oxoglutarate
-
Homo sapiens