Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
expressed in Escherichia coli BL21(DE3) cells and in Sf9 insect cells. ABH3 expressed in insect cells contains no posttranslational modifications but is nevertheless found to be 5fold more active than ABH3 purified from Escherichia coli, suggesting ABH3 may not be correctly folded when purified from Escherichia coli | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Escherichia coli | |
Fe2+ | required for activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
methylated luciferase-mRNA + 2-oxoglutarate + O2 | - |
Homo sapiens | luciferase-mRNA + formaldehyde + succinate + CO2 | - |
? | |
methylated luciferase-mRNA + 2-oxoglutarate + O2 | AlkB is used at a maximal concentration of 0.00083 mM for this reaction since the luciferase-mRNA is degraded when higher AlkB concentrations are used | Escherichia coli | luciferase-mRNA + formaldehyde + succinate + CO2 | - |
? | |
methylated tRNA-Phe + 2-oxoglutarate + O2 | - |
Escherichia coli | tRNA-Phe + formaldehyde + succinate + CO2 | - |
? | |
methylated tRNA-Phe + 2-oxoglutarate + O2 | - |
Homo sapiens | tRNAPhe + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABH3 | - |
Homo sapiens |
AlkB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli | |
2-oxoglutarate | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | ABH3 restores the biological function of mRNA and tRNA inactivated by chemical methylation. A methylation-induced block in translation of an mRNA can be readily relieved by treatment with ABH3 prior to translation. Chemical methylation of tRNA-Phe inhibits aminoacylation and translation, but the inhibition can be reversed by ABH3 | Homo sapiens |
physiological function | AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation. A methylation-induced block in translation of an mRNA can be readily relieved by treatment with AlkB prior to translation. Chemical methylation of tRNA-Phe inhibits aminoacylation and translation, but the inhibition can be reversed by AlkB | Escherichia coli |