Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Papaver somniferum |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain SG13009 | Papaver somniferum |
Protein Variants | Comment | Organism |
---|---|---|
E338G/I340L/L341V/K342E | site-directed mutagenesis, the mutant exhibits demethylase activity exclusively toward codeine, not also toward thebaine like the wild-type enzyme | Papaver somniferum |
R334S/R336S | site-directed mutagenesis, the mutant retains O-demethylase activity toward either thebaine or codeine like the wild-type enzyme | Papaver somniferum |
R334S/R336T | site-directed mutagenesis, the mutant retains O-demethylase activity toward either thebaine or codeine like the wild-type enzyme | Papaver somniferum |
S149M/L151F/S152T | site-directed mutagenesis, the mutant is expressed at low levels, perhaps due to improper folding | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0728 | - |
codeine | pH 7.4, 30°C, wild-type enzyme | Papaver somniferum | |
0.216 | - |
thebaine | pH 7.4, 30°C, wild-type enzyme | Papaver somniferum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + 2-oxoglutarate + O2 | Papaver somniferum | - |
morphine + formaldehyde + succinate + CO2 | - |
? | |
thebaine + 2-oxoglutarate + O2 | Papaver somniferum | - |
oripavine + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type enzyme and mutants from Escherichia coli strain SG13009 by cobalt affinity chromatography | Papaver somniferum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + 2-oxoglutarate + O2 | - |
Papaver somniferum | morphine + formaldehyde + succinate + CO2 | - |
? | |
thebaine + 2-oxoglutarate + O2 | - |
Papaver somniferum | oripavine + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CODM | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the morphinan pathway via codeine of the biosynthesis of benzylisoquinoline alkaloids in Papaver somniferum, overview | Papaver somniferum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000015 | - |
thebaine | pH 7.4, 30°C, wild-type enzyme | Papaver somniferum | |
0.00095 | - |
codeine | pH 7.4, 30°C, wild-type enzyme | Papaver somniferum |