Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.32 extracted from

  • Runguphan, W.; Glenn, W.S.; OConnor, S.E.
    Redesign of a dioxygenase in morphine biosynthesis (2012), Chem. Biol., 19, 674-678.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate
-
Papaver somniferum

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain SG13009 Papaver somniferum

Protein Variants

Protein Variants Comment Organism
E338G/I340L/L341V/K342E site-directed mutagenesis, the mutant exhibits demethylase activity exclusively toward codeine, not also toward thebaine like the wild-type enzyme Papaver somniferum
R334S/R336S site-directed mutagenesis, the mutant retains O-demethylase activity toward either thebaine or codeine like the wild-type enzyme Papaver somniferum
R334S/R336T site-directed mutagenesis, the mutant retains O-demethylase activity toward either thebaine or codeine like the wild-type enzyme Papaver somniferum
S149M/L151F/S152T site-directed mutagenesis, the mutant is expressed at low levels, perhaps due to improper folding Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0728
-
codeine pH 7.4, 30°C, wild-type enzyme Papaver somniferum
0.216
-
thebaine pH 7.4, 30°C, wild-type enzyme Papaver somniferum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
codeine + 2-oxoglutarate + O2 Papaver somniferum
-
morphine + formaldehyde + succinate + CO2
-
?
thebaine + 2-oxoglutarate + O2 Papaver somniferum
-
oripavine + formaldehyde + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Papaver somniferum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type enzyme and mutants from Escherichia coli strain SG13009 by cobalt affinity chromatography Papaver somniferum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
codeine + 2-oxoglutarate + O2
-
Papaver somniferum morphine + formaldehyde + succinate + CO2
-
?
thebaine + 2-oxoglutarate + O2
-
Papaver somniferum oripavine + formaldehyde + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
CODM
-
Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Papaver somniferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Papaver somniferum

General Information

General Information Comment Organism
metabolism the enzyme is involved in the morphinan pathway via codeine of the biosynthesis of benzylisoquinoline alkaloids in Papaver somniferum, overview Papaver somniferum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000015
-
thebaine pH 7.4, 30°C, wild-type enzyme Papaver somniferum
0.00095
-
codeine pH 7.4, 30°C, wild-type enzyme Papaver somniferum