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Literature summary for 1.14.11.27 extracted from

  • Ishimura, A.; Minehata, K.; Terashima, M.; Kondoh, G.; Hara, T.; Suzuki, T.
    Jmjd5, an H3K36me2 histone demethylase, modulates embryonic cell proliferation through the regulation of Cdkn1a expression (2012), Development, 139, 749-759.
    View publication on PubMed
Search for more literature for 1.14.11.27

Cloned(Commentary)

Cloned (Comment) Organism
gene Jmjd5, FLAG-His6 tagged mutant H319A is cloned into the pDON-5 Neo plasmid to produce retroviruses, shRNA-expressing retroviruses are used for silencing of the enzyme, expression of C-terminally His-tagged JMJD5 in Escherichia coli, quantitative reverse transcription PCR expression analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
H319A a catalytically inactive mutant Mus musculus
additional information generation of Jmjd5-deficient mice Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Mus musculus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone H3-N6,N6-dimethyl-L-lysine36 + 2-oxoglutarate + O2 Mus musculus
-
 histone H3-N6-methyl-L-lysine36 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Mus musculus
-
embryonic fibroblast
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H3-N6,N6-dimethyl-L-lysine36 + 2-oxoglutarate + O2
-
 Mus musculus histone H3-N6-methyl-L-lysine36 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
H3K36me2 histone demethylase
-
 Mus musculus
JMJD5
-
 Mus musculus
KDM8
-
 Mus musculus

General Information

General Information Comment Organism
evolution JMJD5/KDM8 is a member of the JmjC family Mus musculus
malfunction Jmjd5-/- embryos show severe growth retardation, resulting in embryonic lethality at the mid-gestation stage, Cdkn1a expression is upregulated in Jmjd5neo/neo MEFs and Jmjd5-/- embryos, which is responsible for the growth defects. phenotypes, overview. Jmjd5neo/neo hypomorphic mouse embryonic fibroblasts proliferate more slowly than wild-type Mus musculus
additional information nuclear protein Jmjd5 or Kdm8 is a histone lysine demethylase that contains a JmjC domain in the C-terminal region Mus musculus
physiological function Jmjd5 is involved in the maintenance of H3K36me2 at the Cdkn1a locus Mus musculus