Literature summary for 1.14.11.26 extracted from

Lloyd, M.D.; Lipscomb, S.J.; Hewitson, K.S.; Hensgens, C.M.H.; Baldwin, J.E.; Schofield, C.J.
Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase (2004), J. Biol. Chem., 279, 15420-15426.

Search for more literature for 1.14.11.26

Protein Variants

Protein Variants	Comment	Organism
M306I	hydroxylation reaction of deacetoxycephalosporin C, EC 1.14.11.26, is abolished, 59% of wild-type ring expansion activity	Acremonium chrysogenum
additional information	truncation of C-terminus to residue 310, 2fold enhancement of ring expansion reaction of penicillin G. Double mutant with truncation at residue 310 and M306I, selective catalyzation of ring expansion. Triple mutant with truncation at residue 310, M306I and N305L, selective catalization of ring expansion with improved kinetic parameters	Acremonium chrysogenum
N305L	107% of wild-type ring expansion activity, 85% of wild-type hydroxylation activity	Acremonium chrysogenum
W82A	5.5% of wild-type ring expansion activity, 71% of wild-type hydroxylation activity	Acremonium chrysogenum
W82A	ring expansion reaction of EC 1.14.20.1 is reduced	Acremonium chrysogenum
W82S	44% of wild-type ring expansion activity, 18% of wild-type hydroxylation activity	Acremonium chrysogenum

Organism

Organism	UniProt	Comment	Textmining
Acremonium chrysogenum	-	catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-aminodeacetoxycephalosporanic acid + 2-oxoglutarate + O2	-	Acremonium chrysogenum	7-aminodeacetylcephalosporin + succinate + CO2	-	?
cephalexin + 2-oxoglutarate + O2	-	Acremonium chrysogenum	? + succinate + CO2	-	?
deacetoxycephalosporin C + 2-oxoglutarate + O2	-	Acremonium chrysogenum	deacetylcephalosporin C + succinate + CO2	-	?
phenylacetyl-7-aminodeacetoxycephalosporanic acid + 2-oxoglutarate + O2	-	Acremonium chrysogenum	phenylacetyl-7-aminodeacetylcephalosporin + succinate + CO2	-	?

Subunits

Subunits	Comment	Organism
More	structural model of enzyme	Acremonium chrysogenum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)