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Literature summary for 1.14.11.20 extracted from

  • De Carolis, E.; De Luca, V.
    Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus (1993), J. Biol. Chem., 268, 5504-5511.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid
-
Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
CO 7.5 mM, 50% inhibition Catharanthus roseus
desacetylvindoline product inhibition, noncompetitive vs. 2-oxoglutarate Catharanthus roseus
succinate product inhibition, competitive vs. 2-oxoglutarate, noncompetitive vs. desacetoxyvindoline Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000003
-
desacetoxyvindoline
-
Catharanthus roseus
0.0085
-
Fe2+
-
Catharanthus roseus
0.045
-
O2
-
Catharanthus roseus
0.045
-
2-oxoglutarate
-
Catharanthus roseus
0.2
-
ascorbate
-
Catharanthus roseus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44700
-
1 * 44700, SDS-PAGE Catharanthus roseus
45000
-
-
Catharanthus roseus

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-100, Green 19-agarose, hydroxylapatite, 2-oxoglutarate-Sepharose, Mono-Q Catharanthus roseus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00517
-
-
Catharanthus roseus

Subunits

Subunits Comment Organism
monomer 1 * 44700, SDS-PAGE Catharanthus roseus

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
Catharanthus roseus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.115
-
deacetylvindoline
-
Catharanthus roseus
9
-
succinate
-
Catharanthus roseus