Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | mainly stimulats P4HA1 | Mus musculus | |
LY83583 | might increase the enzymatic activity of prolyl 4-hydroxylases to increase type I collagen maturation. LY83583 activated prolyl 4-hydrolases differed from ascorbate-activated prolyl 4-hydroxylase in two aspects: (1) LY83583 activats both P4HA1 and P4HA2 involved in collagen maturation whereas ascorbate mainly stimulats P4HA1 in collagen maturation, (2) LY83583 does not induce N259 glycosylation on P4HA1 as ascorbate does | Mus musculus | |
LY83583 | might increase the enzymatic activity of prolyl 4-hydroxylases to increase type I collagen maturation. LY83583 activated prolyl 4-hydrolases differs from ascorbate-activated prolyl 4-hydroxylase in two aspects: (1) LY83583 activats both P4HA1 and P4HA2 involved in collagen maturation whereas ascorbate mainly stimulats P4HA1 in collagen maturation, (2) LY83583 does not induce N259 glycosylation on P4HA1 as ascorbate does | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) pLysS cells | Phytophthora capsici |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion method | Phytophthora capsici |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+-dependent enzyme | Phytophthora capsici |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 | Mus musculus | - |
procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | Phytophthora capsici | - |
procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | Phytophthora capsici LT1534 | - |
procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q60715 | prolyl 4-hydroxylase subunit alpha-1 | - |
Mus musculus | Q60716 | prolyl 4-hydroxylase subunit alpha-2 | - |
Phytophthora capsici | A0A4P1LYH8 | - |
- |
Phytophthora capsici LT1534 | A0A4P1LYH8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N259 glycosylation | Mus musculus |
Purification (Comment) | Organism |
---|---|
- |
Phytophthora capsici |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic fibroblast | - |
Mus musculus | - |
mycelium | - |
Phytophthora capsici | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 | - |
Mus musculus | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | - |
Phytophthora capsici | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | the binding affinity of 2-oxoglutarate to PcP4H1 is very low | Phytophthora capsici | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | - |
Phytophthora capsici LT1534 | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | the binding affinity of 2-oxoglutarate to PcP4H1 is very low | Phytophthora capsici LT1534 | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P4ha1 | - |
Mus musculus |
P4ha2 | - |
Mus musculus |
prolyl 4-hydroxylase | - |
Mus musculus |
prolyl 4-hydroxylase | - |
Phytophthora capsici |
Organism | Comment | Expression |
---|---|---|
Phytophthora capsici | in mycelia, all P4Hs are downregulated in response to hypoxia, but the expression of PcP4H1 is most affected. Pc4H1 is upregulated more than 110fold at the onset of infection, and Pc4H5 is upregulated 7fold, while the expression of other P4Hs is unchanged | up |
General Information | Comment | Organism |
---|---|---|
malfunction | knockout of either P4ha1 or P4ha2 greatly reduces LY83583-stimulated type I collagen maturation whereas silencing of both P4ha1 and P4ha2 completely blocks LY83583-induced type I collagen maturation | Mus musculus |
physiological function | prolyl 4-hydroxylase play central roles in the collagen stabilization, hypoxia sensing, and translational regulation in eukaryotes | Phytophthora capsici |