Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | PHD activity requires iron and ascorbate as cofactors | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | stable knockdown of PHDs in A375 cells does not affect cell proliferation, overview. Sections from PHD3 knockdown xenografts show little increase in cleaved caspase-3 staining after treatment with 2-oxoglutarate | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | PHD activity requires iron and ascorbate as cofactors | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 | Homo sapiens | - |
procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-375 cell | - |
Homo sapiens | - |
A-431 cell | - |
Homo sapiens | - |
HCT-116 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 | - |
Homo sapiens | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PHD1 | - |
Homo sapiens |
PHD2 | - |
Homo sapiens |
PHD3 | - |
Homo sapiens |
prolyl hydroxylase-3 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | 2-oxoglutarate-mediated activation of PHD3 is required for tumor suppression, while PHD2 and HIF1alpha are not required, 2-oxoglutarate does not significantly inhibit tumor growth in PHD3 knockdown xenograft tumors. Reactivating PHD2 in A375 cells has no long-term role in inhibiting tumor growth | Homo sapiens |
physiological function | PHD2 is the major regulator of HIF1alpha stability | Homo sapiens |