Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-pyridine dicarboxylate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
0.5 | - |
PEG-Gly-Tyr-4-fluoroproline-GlyOEt | pH 7.8, 22°C, recombinant enzyme, fluoride-based assay | Homo sapiens | |
1.2 | - |
PEG-Gly-Tyr-4-fluoroproline-GlyOEt | pH 7.8, 22°C, recombinant enzyme, HPLC-based assay | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | P4H is a nonheme iron dioxygenase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 | Homo sapiens | - |
procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-4-fluoroproline + 2-oxoglutarate + O2 | development and evaluation of an assay method that continuously and directly detects turnover of the proline-containing substrate. The assay is based on (2S,4S)-4-fluoroproline, a proline analogue that is transformed into (2S)-4-oxoproline and inorganic fluoride by P4H using a fluoride ion-selective electrode for detection | Homo sapiens | (2S)-4-oxoproline + fluoride | - |
? | |
PEG-Gly-Tyr-4-fluoroproline-GlyOEt + 2-oxoglutarate + O2 | - |
Homo sapiens | ? | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | - |
Homo sapiens | procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | prolyl 4-hydroxylase is a nonheme iron dioxygenase that catalyzes the posttranslational hydroxylation of (2S)-Pro residues in protocollagen strands | Homo sapiens | procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P4H | - |
Homo sapiens |
prolyl 4-hydroxylase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
PEG-Gly-Tyr-4-fluoroproline-GlyOEt | pH 7.8, 22°C, recombinant enzyme, fluoride-based assay | Homo sapiens | |
6 | - |
PEG-Gly-Tyr-4-fluoroproline-GlyOEt | pH 7.8, 22°C, recombinant enzyme, HPLC-based assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | P4H is a nonheme iron dioxygenase that catalyzes the posttranslational hydroxylation of (2S)-Pro residues in protocollagen strands. The resulting (2S,4R)-4-hydroxyproline residues are essential for the folding, secretion, and stability of the collagen triple helix | Homo sapiens |