Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.2 extracted from

  • Keskiaho, K.; Kukkola, L.; Page, A.P.; Winter, A.D.; Vuoristo, J.; Sormunen, R.; Nissi, R.; Riihimaa, P.; Myllyharju, J.
    Characterization of a novel Caenorhabditis elegans prolyl 4-hydroxylase with a unique substrate specificity and restricted expression in the pharynx and excretory duct (2008), J. Biol. Chem., 283, 10679-10689.
    View publication on PubMed
Search for more literature for 1.14.11.2

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of PHY-4.1, product of alpha subunit-like gene Y43F8B.4, and beta-subunit protein-disulfide isomerase PDI2, in Sf9 cell Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
additional information co-expression of PHY-4.1, product of alpha subunit-like gene Y43F8B.4, and beta-subunit protein-disulfide isomerase PDI2 results in active (PHY-4.1)2(PDI2)2 tetramers and in PHY-4.1/PDI2 dimers. Contrary to alpha subunits PHY-1, PHY-2, PHY-3, tetramers containing PHY-4.1 are able to hydroxylate poly(L-Pro) and other proline-rich peptides Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 2-oxoglutarate complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans
0.11
-
 poly(L-Pro) Mr 30000-40000 da, complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans
0.22
-
 poly(L-Pro) Mr 5000-10000 Da, complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans
0.25
-
 (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans
2.5
-
 (L-Pro-L-Pro-Gly)10 L-proline complex of recombinant subunits PHY-4.1 and PDI2 Caenorhabditis elegans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ complex of recombinant subunits PHY-4.1 and PDI2, Km value 0.01 mM Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 PAGE Caenorhabditis elegans
180000
-
 PAGE Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
 PHY-4.1, product of alpha subunit-like gene Y43F8B.4
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant alpha subunit PHY4.1 is partly N-glycosylated Caenorhabditis elegans
proteolytic modification sequence contains a GP67 signal peptide Caenorhabditis elegans

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
(L-Pro-L-Pro-Gly)10 L-proline + 2-oxoglutarate + O2 44.8% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline Caenorhabditis elegans (L-Pro-L-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
L-Ala-(L-Pro)4-Gly-L-Ile-L-Pro-Gly-L-Tyr-(L-Pro)2-L-Ala-(L-Pro)4-Gly-L-Val-Gly-(L-Pro)4-L-Gln-Gly L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans L-Ala-(L-Pro)4-Gly-L-Ile-L-Pro-Gly-L-Tyr-(L-Pro)2-L-Ala-(L-Pro)4-Gly-L-Val-Gly-(L-Pro)4-L-Gln-Gly trans-4-hydroxy-L-proline + succinate + CO2 peptide substrate representing proline-rich proteins expressed in the pharyngeal gland cells. 380% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline ?
L-Pro-L-Ile-(L-Pro)7-L-Leu-L-Pro-L-Gln-L-Asn-L-Leu-L-Ser-Gly-L-Ala-L-Pro L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans L-Pro-L-Ile-(L-Pro)7-L-Leu-L-Pro-L-Gln-L-Asn-L-Leu-L-Ser-Gly-L-Ala-L-Pro trans-4-hydroxy-L-proline + succinate + CO2 peptide substrate representing proline-rich proteins expressed in the pharyngeal gland cells. 610% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline ?
poly(L-Pro) + 2-oxoglutarate + O2 Mr 30000-40000 Da, 15% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline Caenorhabditis elegans poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 ?
poly(L-Pro) + 2-oxoglutarate + O2 Mr 5000-10000 Da, 5, 24% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline Caenorhabditis elegans poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer and tetramer, 1 * alpha subunit PHY4.1 + 1 * beta subunit PDI2, SDS-PAGE of recombinant protein Caenorhabditis elegans
tetramer and dimer, 2 * alpha subunit PHY4.1 + 2 * beta subunit PDI2, SDS-PAGE of recombinant protein Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
ascorbate complex of recombinant subunits PHY-4.1 and PDI2, Km value 0.04 mM Caenorhabditis elegans