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Literature summary for 1.14.11.2 extracted from

  • Koski, M.K.; Hieta, R.; Boellner, C.; Kivirikko, K.I.; Myllyharju, J.; Wierenga, R.K.
    The active site of an algal prolyl 4-hydroxylase has a large structural plasticity (2007), J. Biol. Chem., 282, 37112-37123.
    View publication on PubMed
Search for more literature for 1.14.11.2

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of apoenzyme at 1.93 and 2.90 A resolution, complexed with the competitive inhibitor Zn2+, and with Zn2+ and pyridine 2,4-dicarboxylate at 1.85 A resolution. The structures reveal the double-stranded alpha-helix core fold typical for 2-oxoglutarate dioxygenases. The catalytic site is at the center of an extended shallow groove lined by two flexible loops Chlamydomonas reinhardtii

Protein Variants

Protein Variants Comment Organism
E127A very low catalytic activity Chlamydomonas reinhardtii
H245A no catalytic activity Chlamydomonas reinhardtii
Q130A almost 2-fold increase in Km for poly(L-Pro) Chlamydomonas reinhardtii
R161A no catalytic activity Chlamydomonas reinhardtii
R93A no catalytic activity Chlamydomonas reinhardtii
S95A no catalytic activity Chlamydomonas reinhardtii
W243A Km values similar to wild-type, 10-fold decrease in kcat Chlamydomonas reinhardtii
W99A 2.5-fold increase in Km for poly(L-Pro) Chlamydomonas reinhardtii
Y134F 6-fold increase in Km for 2-oxoglutarate, decrease in kcat Chlamydomonas reinhardtii
Y140A no catalytic activity Chlamydomonas reinhardtii
Y168F 3-4-fold increase in Km for 2-oxoglutarate, decrease in kcat Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 2-oxoglutarate mutant W99A Chlamydomonas reinhardtii
0.14
-
 2-oxoglutarate mutant W243A Chlamydomonas reinhardtii
0.15
-
 2-oxoglutarate mutant Q130A Chlamydomonas reinhardtii
0.16
-
 2-oxoglutarate wild-type Chlamydomonas reinhardtii
0.25
-
 poly(L-Pro) wild-type Chlamydomonas reinhardtii
0.27
-
 poly(L-Pro) mutant Y168F Chlamydomonas reinhardtii
0.28
-
 poly(L-Pro) mutant W243A Chlamydomonas reinhardtii
0.28
-
 poly(L-Pro) mutant Y134F Chlamydomonas reinhardtii
0.46
-
 poly(L-Pro) mutant Q130A Chlamydomonas reinhardtii
0.57
-
 2-oxoglutarate mutant Y168F Chlamydomonas reinhardtii
0.65
-
 poly(L-Pro) mutant W99A Chlamydomonas reinhardtii
1
-
 2-oxoglutarate mutant Y134F Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(L-Pro) + 2-oxoglutarate + O2 Mr of poly(L-Pro) is about 5000 Chlamydomonas reinhardtii poly(4-hydroxyproline) + succinate + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 poly(L-Pro) mutant Y134F Chlamydomonas reinhardtii
6
-
 poly(L-Pro) mutant W243A Chlamydomonas reinhardtii
20
-
 poly(L-Pro) mutant W99A Chlamydomonas reinhardtii
30
-
 poly(L-Pro) mutant Y168F Chlamydomonas reinhardtii
35
-
 poly(L-Pro) mutant Q130A Chlamydomonas reinhardtii
70
-
 poly(L-Pro) wild-type Chlamydomonas reinhardtii