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Literature summary for 1.14.11.2 extracted from

  • Eriksson, M.; Myllyharju, J.; Tu, H.; Hellman, M.; Kivirikko, K.I.
    Evidence for 4-hydroxyproline in viral proteins. Characterization of a viral prolyl 4-hydroxylase and its peptide substrates (1999), J. Biol. Chem., 274, 22131-22134.
    View publication on PubMed
Search for more literature for 1.14.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, the genome of virus-1 encodes a 242-amino acid polypeptide that shows a distinct amino acid sequence similarity to the C-terminal half of the catalytic alpha subunit of animal enzymes, the genome contains many open reading frames for proteins with proline-rich repeats Paramecium bursaria Chlorella virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2-oxoglutarate recombinant enzyme Paramecium bursaria Chlorella virus 1
0.02
-
 (Pro-Ala-Pro-Lys)10 recombinant enzyme Paramecium bursaria Chlorella virus 1
0.02
-
 (Ser-Pro-Lys-Pro-Pro)5 recombinant enzyme Paramecium bursaria Chlorella virus 1
0.05
-
 (Pro-Ala-Pro-Lys)5 recombinant enzyme Paramecium bursaria Chlorella virus 1
0.1
-
 poly(L-proline) MW: 40000, recombinant enzyme Paramecium bursaria Chlorella virus 1
0.31
-
 (L-Pro-L-Ala-L-Pro-L-Lys)3 recombinant enzyme Paramecium bursaria Chlorella virus 1
0.5
-
 poly(L-proline) MW: 13000, recombinant enzyme Paramecium bursaria Chlorella virus 1
0.95
-
 (L-Pro-L-Ala-L-Pro-L-Lys)2 recombinant enzyme Paramecium bursaria Chlorella virus 1
1
-
 (Pro-Glu-Pro-Pro-Ala)5 recombinant enzyme Paramecium bursaria Chlorella virus 1
2.9
-
 (L-Pro-L-Pro-Gly)10 recombinant enzyme Paramecium bursaria Chlorella virus 1
4.8
-
 L-Pro-L-Ala-L-Pro-L-Lys recombinant enzyme Paramecium bursaria Chlorella virus 1
8.6
-
 Lys-Pro-Ala recombinant enzyme Paramecium bursaria Chlorella virus 1

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ recombinant enzyme: Km 0.0004 mM Paramecium bursaria Chlorella virus 1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27200
-
 recombinant enzyme, calculated with the N-terminal histidine tag and the thrombin cleavage site Paramecium bursaria Chlorella virus 1
30000
-
 recombinant enzyme, gel filtration Paramecium bursaria Chlorella virus 1

Organism

Organism UniProt Comment Textmining
Paramecium bursaria Chlorella virus 1
-
 virus-1, eukaryotic algal virus
-

Purification (Commentary)

Purification (Comment) Organism
using Ni2+-chelate affinity chromatography Paramecium bursaria Chlorella virus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Pro-L-Ala-L-Pro-L-Lys)2 + 2-oxoglutarate + O2
-
 Paramecium bursaria Chlorella virus 1 (L-Pro-L-Ala-L-Pro-L-Lys)2-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(L-Pro-L-Ala-L-Pro-L-Lys)3 + 2-oxoglutarate + O2
-
 Paramecium bursaria Chlorella virus 1 (L-Pro-L-Ala-L-Pro-L-Lys)3-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2
-
 Paramecium bursaria Chlorella virus 1 (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(Pro-Ala-Pro-Lys)n + 2-oxoglutarate + O2 n: 1-10, recombinant enzyme, (Pro-Ala-Pro-Lys)10 is a particularly good substrate Paramecium bursaria Chlorella virus 1 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
(Pro-Glu-Pro-Pro-Ala)5 + 2-oxoglutarate + O2 recombinant enzyme Paramecium bursaria Chlorella virus 1 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 recombinant enzyme Paramecium bursaria Chlorella virus 1 (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Paramecium bursaria Chlorella virus 1 (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Ser-Pro-Lys-Pro-Pro)5 + 2-oxoglutarate + O2 recombinant enzyme, (Ser-Pro-Lys-Pro)5 is a particularly good substrate Paramecium bursaria Chlorella virus 1 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
L-Pro-L-Ala-L-Pro-L-Lys + 2-oxoglutarate + O2
-
 Paramecium bursaria Chlorella virus 1 L-Pro-L-Ala-L-Pro-L-Lys-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
Lys-Pro-Ala + 2-oxoglutarate + O2 recombinant enzyme Paramecium bursaria Chlorella virus 1 Lys-4-hydroxyproline-Ala + succinate + CO2
-
 ?
poly(L-Pro) + 2-oxoglutarate + O2 recombinant enzyme Paramecium bursaria Chlorella virus 1 poly(4-hydroxyproline) + succinate + CO2
-
 ?
poly(L-proline) + 2-oxoglutarate + O2 poly(L-proline) with Mr 40000 Paramecium bursaria Chlorella virus 1 poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r
poly(L-proline) + 2-oxoglutarate + O2 poly(L-proline) with Mr 13000 Paramecium bursaria Chlorella virus 1 poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r

Subunits

Subunits Comment Organism
monomer recombinant enzyme, SDS-PAGE Paramecium bursaria Chlorella virus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Paramecium bursaria Chlorella virus 1

Cofactor

Cofactor Comment Organism Structure
ascorbate recombinant enzyme: Km 0.3 mM Paramecium bursaria Chlorella virus 1