Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Rattus norvegicus | |
bovine serum albumin | activation | Lumbricus terrestris | |
dithiothreitol | activation | Rattus norvegicus | |
dithiothreitol | activation | Lumbricus terrestris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Rattus norvegicus | |
Fe2+ | - |
Lumbricus terrestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lumbricus terrestris | - |
earthworm | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and calcium phosphate gel fractionation | Lumbricus terrestris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cuticle | - |
Lumbricus terrestris | - |
skin | new born | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
- |
Lumbricus terrestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Gly-Ala-Pro)n + 2-oxoglutarate + O2 | less than 10% relative activity with respect to (Gly-Pro-Ala)n | Lumbricus terrestris | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
(Gly-Ala-Pro)n + 2-oxoglutarate + O2 | active substrate | Rattus norvegicus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
(Gly-Pro-Ala)n + 2-oxoglutarate + O2 | poor substrate | Rattus norvegicus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
(Gly-Pro-Ala)n + 2-oxoglutarate + O2 | the best substrate | Lumbricus terrestris | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
(Pro-Gly-Pro)n + 2-oxoglutarate + O2 | active substrate | Rattus norvegicus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
(Pro-Gly-Pro)n + 2-oxoglutarate + O2 | active substrate | Lumbricus terrestris | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
additional information | - |
Rattus norvegicus | ? | - |
? | |
additional information | the enzyme clearly prefers X position proline to Y position proline in the sequences of (Gly-Pro-Ala)n versus (Gly-Ala-Pro)n | Lumbricus terrestris | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Rattus norvegicus | |
2-oxoglutarate | - |
Lumbricus terrestris | |
ascorbate | - |
Rattus norvegicus | |
ascorbate | - |
Lumbricus terrestris |