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Literature summary for 1.14.11.2 extracted from
- Isolation and characterization of prolyl hydroxylase from Chlamydomonas reinhardii (1986), Planta, 169, 238-244.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | activation | Chlamydomonas reinhardtii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | at 0.5 mM | Chlamydomonas reinhardtii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
poly(L-Pro) | MW 7000 | Chlamydomonas reinhardtii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | loosely associated to membrane | Chlamydomonas reinhardtii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 60000 + 2 * 65000, SDS-PAGE | Chlamydomonas reinhardtii |
| 60000 | - |
2 * 60000 + 2 * 65000 | Chlamydomonas reinhardtii |
| 65000 | - |
2 * 60000 + 2 * 65000, SDS-PAGE | Chlamydomonas reinhardtii |
| 65000 | - |
2 * 60000 + 2 * 65000 | Chlamydomonas reinhardtii |
| 250000 | - |
native gel electrophoresis | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ion-exchange chromatography on DEAE-cellulose, affinity chromatography with poly-L-proline coupled to tresyl-activated Sepharose-4B and gel filtration | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000066 | - |
purified enzyme, 6 M-urea-elution and dialysis | Chlamydomonas reinhardtii |
| 0.000786 | - |
purified enzyme, poly-L-proline-eluted | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Pro-Pro-Gly)n + 2-oxoglutarate + O2 | - |
Chlamydomonas reinhardtii | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | - |
? | |
| bradykinin + 2-oxoglutarate + O2 | - |
Chlamydomonas reinhardtii | 4-hydroxyproline containing bradykinin | - |
? | |
| poly(L-Pro) + 2-oxoglutarate + O2 | MW 7000 | Chlamydomonas reinhardtii | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 60000 + 2 * 65000, SDS-PAGE | Chlamydomonas reinhardtii |
| tetramer | 2 * 60000 + 2 * 65000 | Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Chlamydomonas reinhardtii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Chlamydomonas reinhardtii | |
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