Any feedback?
Literature summary for 1.14.11.2 extracted from
- Characterization of a low-relative-molecular-mass prolyl 4-hydroxylase from the green alga Chlamydomonas reinhardii (1987), Biochem. J., 241, 483-490.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | activation | Chlamydomonas reinhardtii | |
| catalase | activation | Chlamydomonas reinhardtii | |
| D-isoascorbate | can partially replace ascorbate | Chlamydomonas reinhardtii |  |
| dithiothreitol | activation | Chlamydomonas reinhardtii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoadipate | - |
Chlamydomonas reinhardtii | |
| 2-oxosuccinate | i.e. oxaloacetate | Chlamydomonas reinhardtii | |
| 3,4-dihydroxybenzoate | - |
Chlamydomonas reinhardtii | |
| Benzene 1,3-dicarboxylate | - |
Chlamydomonas reinhardtii | |
| Glutarate | - |
Chlamydomonas reinhardtii | |
| Pyridine 2,4-dicarboxylate | - |
Chlamydomonas reinhardtii | |
| Pyridine 2,5-dicarboxylate | - |
Chlamydomonas reinhardtii | |
| pyridine 2-carboxylate | - |
Chlamydomonas reinhardtii | |
| pyruvate | - |
Chlamydomonas reinhardtii | |
| succinate | - |
Chlamydomonas reinhardtii | |
| Zn2+ | - |
Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
poly(L-Pro) | MW 31000 | Chlamydomonas reinhardtii | |
| 0.011 | - |
poly(L-Pro) | MW 19000 | Chlamydomonas reinhardtii | |
| 0.023 | - |
poly(L-Pro) | MW 7000 | Chlamydomonas reinhardtii | |
| 0.03 | - |
2-oxoglutarate | - |
Chlamydomonas reinhardtii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | Km: 0.01-0.03 mM | Chlamydomonas reinhardtii | |
| Fe2+ | Km: 0.028 mM | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ion-exchange chromatography on DEAE-cellulose and gel filtration | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Pro-Pro-Gly)n + 2-oxoglutarate + O2 | a low hydroxylation rate is found with denatured (Pro-Pro-Gly)10 | Chlamydomonas reinhardtii | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | - |
? | |
| 2-oxoglutarate + O2 + ascorbate | - |
Chlamydomonas reinhardtii | succinate + dehydroascorbate + CO2 + H2O | - |
? | |
| additional information | protocollagen and triple-helical (Pro-Pro-Gly)10 do not serve as substrate. 2-oxoadipate, 2-oxosuccinate or 2-oxovalerate do not replace 2-oxoglutarate | Chlamydomonas reinhardtii | ? | - |
? | |
| poly(L-Pro) + 2-oxoglutarate + O2 | - |
Chlamydomonas reinhardtii | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 6.9 | - |
Chlamydomonas reinhardtii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,6-Isopropylideneascorbate | can replace ascorbate | Chlamydomonas reinhardtii | |
| ascorbate | Km: 0.240 mM | Chlamydomonas reinhardtii | |
| D-isoascorbate | can replace ascorbate | Chlamydomonas reinhardtii | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
Pyridine 2,4-dicarboxylate | - |
Chlamydomonas reinhardtii | |
| 0.02 | - |
Pyridine 2,5-dicarboxylate | - |
Chlamydomonas reinhardtii | |
| 0.05 | - |
pyridine 2-carboxylate | - |
Chlamydomonas reinhardtii | |
| 0.07 | - |
3,4-dihydroxybenzoate | - |
Chlamydomonas reinhardtii | |
| 0.2 | - |
2-oxoadipinate | - |
Chlamydomonas reinhardtii | |
| 0.4 | - |
2-oxosuccinate | - |
Chlamydomonas reinhardtii | |
| 1.5 | - |
Glutarate | - |
Chlamydomonas reinhardtii | |
| 1.6 | - |
succinate | - |
Chlamydomonas reinhardtii | |
| 11 | - |
Benzene 1,3-dicarboxylate | - |
Chlamydomonas reinhardtii | |
| 20 | - |
pyruvate | - |
Chlamydomonas reinhardtii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
