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Literature summary for 1.14.11.2 extracted from
- Elicitor-induced prolyl hydroxylase from French bean (Phaseolus vulgaris). Localization, purification and properties (1985), Biochem. J., 229, 693-699.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | activation at 0.1% v/v | Phaseolus vulgaris |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Phaseolus vulgaris | |
| EDTA | - |
Phaseolus vulgaris | |
| EGTA | - |
Phaseolus vulgaris | |
| Salicylyl hydroxamate | - |
Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
poly(L-Pro) | MW 30000 | Phaseolus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Phaseolus vulgaris | 5783 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Phaseolus vulgaris |
| 300000 | - |
gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
french bean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ion-exchange chromatography on DEAE-cellulose and affinity chromatography on a column of poly-L-proline-Sepharose 4B | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Phaseolus vulgaris |
| 0.0714 | - |
homogenate | Phaseolus vulgaris |
| 0.138 | - |
15000 g pellet | Phaseolus vulgaris |
| 0.162 | - |
microsomal fraction, rough membrane | Phaseolus vulgaris |
| 0.27 | - |
1000 g pellet | Phaseolus vulgaris |
| 0.63 | - |
microsomal fraction | Phaseolus vulgaris |
| 1.668 | - |
microsomal fraction, smooth membrane | Phaseolus vulgaris |
| 2.328 | - |
purified enzyme, 6 M-urea eluted, then dialysed | Phaseolus vulgaris |
| 87.53 | - |
purified enzyme, poly-L-proline-eluted | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(L-Pro) + 2-oxoglutarate + O2 | - |
Phaseolus vulgaris | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Phaseolus vulgaris |
| tetramer | doublet with MW of 65000, SDS-PAGE | Phaseolus vulgaris |
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