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Literature summary for 1.14.11.2 extracted from

  • Cunliffe, C.J.; Franklin, T.J.
    Inhibition of prolyl 4-hydroxylase by hydroxyanthraquinones (1986), Biochem. J., 239, 311-315.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
catalase activation Gallus gallus
dithiothreitol activation Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
2,7,8-trihydroxyanthraquinone 50% inhibition at 0.047 mM, competitive inhibitor with respect to 2-oxoglutarate, non-competitive with regard to ascorbate, uncompetitive with regard to protocollagen. The inhibition is greatly enhanced in the absence of Fe2+, structural requirements for inhibition Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
2 * 60000 + 2 * 64000, SDS-PAGE Gallus gallus
64000
-
2 * 60000 + 2 * 64000, SDS-PAGE Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo leg Gallus gallus
-
leg embryo Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protocollagen + 2-oxoglutarate + O2
-
Gallus gallus 4-hydroxyproline containing protocollagen + succinate + CO2
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 60000 + 2 * 64000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0403
-
2,7,8-trihydroxyanthraquinone
-
Gallus gallus