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Literature summary for 1.14.11.17 extracted from

  • Wetzl, D.; Bolsinger, J.; Nestl, B.; Hauer, B.
    alpha-Hydroxylation of carboxylic acids catalyzed by taurine dioxygenase (2016), ChemCatChem, 8, 1361-1366 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
F206Y the conversions of the improved catalyst increases by at least 140% compared to that of the wild-type enzyme and shows broadened substrate scope Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P37610
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutyric acid + 2-oxoglutarate + O2 activity with mutant enzyme F206Y is 4.7fold higher than activity with wild-type enzyme Escherichia coli D-2-hydroxy-4-aminobutyric acid + succinate + CO2
-
?
5-aminovaleric acid + 2-oxoglutarate + O2 activity with mutant enzyme F206Y is 4.4fold higher than activity with wild-type enzyme Escherichia coli D-2-hydroxy-5-aminovaleric acid + succinate + CO2
-
?
6-aminocaproic acid + 2-oxoglutarate + O2 mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme Escherichia coli D-2-hydroxy-6-aminocaproic acid + succinate + CO2
-
?
alpha-methyl-beta-alanine + 2-oxoglutarate + O2 mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme Escherichia coli 3-amino-2-hydroxy-2-methylpropanoic acid + succinate + CO2
-
?
beta-alanine + 2-oxoglutarate + O2 activity with mutant enzyme F206Y is 2.4fold higher than activity with wild-type enzyme Escherichia coli D-isoserine + succinate + CO2
-
?
butyric acid + 2-oxoglutarate + O2 mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme Escherichia coli 2-hydroxybutyric acid + succinate + CO2
-
?
propionic acid + 2-oxoglutarate + O2 mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme Escherichia coli 2-hydroxypropionic acid + succinate + CO2
-
?
valeric acid + 2-oxoglutarate + O2 mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme Escherichia coli 2-hydroxyvaleric acid + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
TauD
-
Escherichia coli