Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | 50% increase in activity at 0.2-0.8 mM | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inhibits activity by 80-95% at 0.01-0.05 mM | Escherichia coli | |
Cu2+ | inhibits activity by 80-95% at 0.01-0.05 mM | Escherichia coli | |
EDTA | complete inactivation | Escherichia coli | |
Zn2+ | inhibits activity by 80-95% at 0.01-0.05 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
2-oxoglutarate | - |
Escherichia coli | |
0.055 | - |
taurine | - |
Escherichia coli | |
0.145 | - |
MOPS | - |
Escherichia coli | |
0.485 | - |
1,3-dioxo-2-isoindolineethanesulfonic acid | - |
Escherichia coli | |
0.59 | - |
pentanesulfonic acid | - |
Escherichia coli | |
1.49 | - |
butanesulfonic acid | - |
Escherichia coli | |
1.51 | - |
hexanesulfonic acid | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | maximal activation between 0.005 and 0.150 mM | Escherichia coli | |
additional information | Mg2+, Ca2+, Mn2+ or Ni2+ can not replace iron | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32410 | - |
calculation from gene sequence | Escherichia coli |
37400 | - |
estimated by SDS-PAGE | Escherichia coli |
81000 | - |
gel filtration on Superose 6 and Superose 12 HR | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two-step purification from overexpressing Escherichia coli to apparent homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
strain MC4100 grown in sulfate-free minimal medium containing 0.25 mM taurine as sulfur source | Escherichia coli |
1.64 | - |
purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, phosphate buffer, 16% glycerol, 10 weeks, activity increases 4-fold | Escherichia coli |
-20°C, phosphate buffer, without glycerol, 3 weeks, more than 50% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-dioxo-2-isoindolineethanesulfonic acid + 2-oxoglutarate + O2 | - |
Escherichia coli | sulfite + ? + succinate + CO2 | - |
? | |
butanesulfonic acid + 2-oxoglutarate + O2 | - |
Escherichia coli | sulfite + butanal + succinate + CO2 | - |
? | |
hexanesulfonic acid + 2-oxoglutarate + O2 | - |
Escherichia coli | sulfite + hexanal + succinate + CO2 | - |
? | |
MOPS + 2-oxoglutarate + O2 | - |
Escherichia coli | sulfite + ? + succinate + CO2 | - |
? | |
pentanesulfonic acid + 2-oxoglutarate + O2 | - |
Escherichia coli | sulfite + pentanal + succinate + CO2 | - |
? | |
taurine + 2-oxoglutarate + O2 | no substrates are methanesulfonic acid, ethanesulfonic acid, isethionic acid, 2-bromoethanesulfonic acid, L-cysteic acid, sulfosuccinate, 4-aminobenzenesulfonic acid, 2-(4-pyridyl)ethanesulfonic acid, N-phenyltaurine | Escherichia coli | sulfite + aminoacetaldehyde + succinate + CO2 | - |
? | |
taurine + alpha-ketoadipate + O2 | alpha-ketoadipate is less active than 2-oxoglutarate, no activity with pyruvate, alpha-ketobutyrate, alpha-ketovalerate, alpha-ketocaproate, alpha-ketoisovalerate and oxalacetat | Escherichia coli | sulfite + aminoacetaldehyde + pentan-1,5-dioic acid + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37400, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
incubation at 30°C leads to rapid inactivation, effect is enhanced by ascorbate and not due to oxidation of the enzyme-bound ferrous iron | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Escherichia coli |