Literature summary for 1.14.11.13 extracted from

Lo, S.F.; Yang, S.Y.; Chen, K.T.; Hsing, Y.I.; Zeevaart, J.A.; Chen, L.J.; Yu, S.M.
A novel class of gibberellin 2-oxidases control semidwarfism, tillering, and root development in rice (2008), Plant Cell, 20, 2603-2618.

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Application

Application	Comment	Organism
agriculture	breeding plants with reduced height, increased root biomass, normal flowering and seed production by overexpression of C20 gibberellin 2-oxidases: first overexpression of GA2ox9ACT mutant generates semidwarf rice with only slightly reduced grain weight and fertility, increased tiller number (by 22%) compared to wild-type, second overexpression of C20 GA2oxs with defective motif III, such as GA2ox5delta335-341ACT mutant, generates a semidwarf rice variety with reduced grain weight (by 16%) and fertility (by 12%) and twofold increased tiller number, third overexpression of a selected C20 GA2ox gene, such as GA2ox6 with less effect on plant growth under the control of a weak promoter could be beneficial without sacrificing seed production	Oryza sativa

Cloned(Commentary)

Cloned (Comment)	Organism
PCR-amplifiction of cDNA, inserted via pGEM-T Easy cloning vector and pAHC18 into pCAMBIA1301 and transferred into Agrobacterium tumefaciens (strain EHA105) and used to transform rice and tobacco (Nicotiana tabacum), or for activity assays subcloned into pGEX-5X expression vector to transform Escherichia coli BL21(DE3)	Oryza sativa

Protein Variants

Protein Variants	Comment	Organism
additional information	mutants overexpressing GA2ox9ACT, GA2ox5delta335-341ACT, and GA2ox6, motif III of the conserved motifs characteristic for C20 GA2oxs is necessary for C20 GA2ox activity, motif III is present in rice GA2ox5, GA2ox6, and GAsox9	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
gibberellin + 2-oxoglutarate + O2	Oryza sativa	catabolization of biologically active gibberellin	2beta-hydroxygibberellin + succinate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	-	rice cultivar Tainung 67	-

Purification (Commentary)

Purification (Comment)	Organism
Escherichia coli with recombinant enzyme are centrifuged, resuspended in a BugBuster Protein Extraction Reagent, centrifuged, supernatants eluted through a GST-Bind resin with 50 mM Tris buffer, pH 8.0, containing 10 mM reduced glutathione, storage at -80°C	Oryza sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
gibberellin + 2-oxoglutarate + O2	catabolization of biologically active gibberellin	Oryza sativa	2beta-hydroxygibberellin + succinate + CO2	-	?
gibberellin 12 + 2-oxoglutarate + O2	GA2ox5 and GA2ox6	Oryza sativa	gibberellin 110 + succinate + CO2	-	?
gibberellin 53 + 2-oxoglutarate + O2	GA2ox5 and GA2ox6	Oryza sativa	gibberellin 97 + succinate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
C20 GA2ox	-	Oryza sativa
GA2ox5	-	Oryza sativa
GA2ox6	-	Oryza sativa
gibberellin 2-oxidase	4 partially characterized gibberellin 2-oxidases (GA2ox) (GA2ox1 to GA2ox4), 2 uncharacterized GA2oxs (GA2ox5 and GA2ox6), 4 other (GA2oxs GA2ox7 to GA2ox9, GA2ox10 is probably a pseudogene)	Oryza sativa

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Release 2023.1 (January 2023)