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Literature summary for 1.14.11.13 extracted from

  • Smith, V.A.; MacMillan, J.
    The partial purification and characterisation of gibberellin 2beta-hydroxylases from seeds of Pisum sativum (1986), Planta, 167, 9-18.
    View publication on PubMed
Search for more literature for 1.14.11.13

General Stability

General Stability Organism
enzyme is very acid-labile. EDTA has beneficial effect on enzyme stability if Mg2+ is present in storage buffer Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
gibberellin 1 efficient inhibitor of gibberellin 20 2beta-hydroxylation Pisum sativum
Zn2+
-
 Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000069
-
 gibberellin 1
-
 Pisum sativum
0.000299
-
 gibberellin 9
-
 Pisum sativum
0.00155
-
 gibberellin 20
-
 Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activity dependent upon Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 major protein, SDS-PAGE Pisum sativum
44000
-
 gel filtration Pisum sativum
45000
-
 minor protein, SDS-PAGE Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 garden pea, cv. progress No. 9
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Pisum sativum
-
embryo embryonic axes Pisum sativum
-
seed mature and immature seeds Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gibberellin 1 + 2-oxoglutarate + O2
-
 Pisum sativum 2beta-hydroxygibberellin 1 + succinate + CO2
-
 ?
gibberellin 20 + 2-oxoglutarate + O2
-
 Pisum sativum gibberellin 29 + succinate + CO2
-
 ?
gibberellin 4 + 2-oxoglutarate + O2
-
 Pisum sativum gibberellin 34 + succinate + CO2
-
 ?
gibberellin 9 + 2-oxoglutarate + O2
-
 Pisum sativum gibberellin 51 + succinate + CO2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.8
-
 Pisum sativum

pH Range

pH Minimum pH Maximum Comment Organism
6.1 7.8
-
 Pisum sativum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8 7.8 activity is highest at pH 7.0-8.0 and decreases rapidly below pH 7.0, enzyme is unstable when stored below pH 7 or in absence of a thiol reagent Pisum sativum

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate activity dependent upon Pisum sativum
ascorbate activity dependend upon Pisum sativum