Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.11 extracted from

  • Fischer, C.; Kwon, M.; Ro, D.K.; van Belkum, M.J.; Vederas, J.C.
    Isolation, expression and biochemical characterization of recombinant hyoscyamine-6beta-hydroxylase from Brugmansia sanguinea - tuning the scopolamine production (2018), MedChemComm, 9, 888-892 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli fused to a small ubiquitin-like modifier protein (SUMO) bearing an N-terminal His6-tag Brugmansia sanguinea

Protein Variants

Protein Variants Comment Organism
additional information truncation of an H6H enzyme flexible N-terminal region yields an active and stable yet more compact enzyme version Brugmansia sanguinea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
 L-hyoscyamine pH 7.6, 34°C Brugmansia sanguinea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38768
-
 calculated from sequence, predominantly exists as monomer (90%) Brugmansia sanguinea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2 Brugmansia sanguinea
-
 (6S)-hydroxyhyoscyamine + succinate + CO
-
 ?

Organism

Organism UniProt Comment Textmining
Brugmansia sanguinea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
SUMO-fusion procedure Brugmansia sanguinea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2
-
 Brugmansia sanguinea (6S)-hydroxyhyoscyamine + succinate + CO
-
 ?

Subunits

Subunits Comment Organism
monomer x * 38768, calculated from sequence Brugmansia sanguinea

Synonyms

Synonyms Comment Organism
H6H
-
 Brugmansia sanguinea
hyoscyamine-6beta-hydroxylase
-
 Brugmansia sanguinea

pI Value

Organism Comment pI Value Maximum pI Value
Brugmansia sanguinea calculated from sequence
-
 4.75

General Information

General Information Comment Organism
physiological function rate-limiting key enzymes in the biosynthesis of scopolamine Brugmansia sanguinea