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Literature summary for 1.13.99.1 extracted from

  • Nayak, B.; Kondeti, V.K.; Xie, P.; Lin, S.; Viswakarma, N.; Raparia, K.; Kanwar, Y.S.
    Transcriptional and post-translational modulation of myo-inositol oxygenase by high glucose and related pathobiological stresses (2011), J. Biol. Chem., 286, 27594-27611.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.99.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
S24A/S205A/S218A/T29A/T69A mutated putative PKA phosphorylation sites: mutant is also phosphorylated but to a minor degree Mus musculus
S24A/S205A/S218A/T29A/T69A/S64A/T40A/T49A/T69A/T253A mutated putative PKA and PKC phosphorylation sites: mutant is also phosphorylated but to a minor degree Mus musculus
S64A/T40A/T49A/T69A/T253A mutated putative PKC phosphorylation sites: mutant is also phosphorylated but to a minor degree Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein mutants with substituted phosphorylation sites have a minimal increase in activity. Treatment of cells with PKC, PKA, and PDK1 kinase activators increased activity, whereas inhibitors reduced it. Protein kinases A, C, and PDK1 are capable of phosphorylating mouse in both prokaryotic and eukaryotic systems. Using deletion constructs it is shown that the N-terminus contains the critical phosphorylation sites that are highly relevant to the functionality of the protein Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol + O2
-
 Mus musculus D-glucuronic acid + H2O
-
 ?

Synonyms

Synonyms Comment Organism
Myo-inositol oxygenase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Mus musculus

Expression

Organism Comment Expression
Mus musculus antioxidants N-acetylcysteine, beta-naphthoflavone, and tertiary butyl hydroquinone reduces MIOX expression down
Mus musculus high glucose levels, exposure of cells to oxidants H2O2 and methylglyoxal up-regulates MIOX expression up